The effect of growth factors on the proliferation of human endometrial stromal cells in culture

Mary Hammond, M G Hammond, Hammond MG, Sung-Tack Oh, S. T. Oh, Oh ST, Anners J, Joyce Anners, J Anners, Surrey ES, Eric S. Surrey, E. S. Surrey, Jouko Halme, J Halme, Halme J
American journal of obstetrics and gynecology · 1993 · vol. 168(4) , pp. 1131–1136 · doi:10.1016/0002-9378(93)90356-n · PMID:8475958 · W2061722948
Lab / animal OA: closed CC0 ⤵ 59 in-corpus citations
View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-06-08 ⓘ

This paper investigated the impact of various growth factors on the proliferation of human endometrial stromal cells cultured in vitro.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

OBJECTIVE: Development of ectopic implants of endometriosis is associated with both an inflammatory response by macrophages and endometrial stromal cell proliferation. Macrophages are capable of releasing a variety of inflammatory mediators, including growth factors. To assess the impact of such factors on endometrial tissue, we have studied the effects of recombinant growth factors, fibroblast growth factor, epidermal growth factor, transforming growth factor-alpha, and inflammation mediators transforming growth factor-beta, and tumor necrosis factor-alpha on human endometrial stromal cell proliferation. STUDY DESIGN: Increasing concentrations of these compounds were added to cultures of primary, secondary, and long-term stromal cells and the cells were harvested at 24, 48, and 72 hours. RESULTS: Epidermal growth factor, transforming growth factor-alpha, transforming growth factor-beta, and fibroblast growth factor induced a statistically significant, dose-dependent increase in stromal cell thymidine uptake of 1.5- to fivefold. The cytokine tumor necrosis factor had no effect alone, but the combination of fibroblast growth factor and tumor necrosis factor had a synergistic effect, increasing cell proliferation 25% to 84% over fibroblast growth factor alone. CONCLUSION: The stromal cell response to a wide range of cell growth effectors and the potential of mediators like tumor necrosis factor-alpha to synergize suggest that such macrophage-secretory products may contribute to proliferation of endometrial implants in vivo.

My notes (saved in your browser only)

MeSH descriptors

Endometrium Growth Substances Cell Division Cell Division Cell Division Cells, Cultured Cells, Cultured Drug Synergism Drug Synergism Endometrium Endometrium Epidermal Growth Factor Epidermal Growth Factor Epidermal Growth Factor Female Female Fibroblast Growth Factors Fibroblast Growth Factors Fibroblast Growth Factors Growth Substances

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (26)

Cited by (50)

Source provenance

europepmc
last seen: 2026-10-13T06:10:10.580696+00:00
openalex
last seen: 2026-05-11T06:41:50.100108+00:00
pubmed
last seen: 2026-10-08T21:59:37.277143+00:00
unpaywall
last seen: 2026-10-13T06:25:51.201866+00:00
License: CC0 · commercial use OK