Effect of Peritoneal Fluid From Endometriosis Patients on Endometrial Stromal Cell Proliferation In Vitro

Eric S. Surrey, E. S. Surrey, Surrey ES, Halme J, J Halme, Jouko Halme
Obstetrics and gynecology · 1990 · vol. 76(5) , pp. 792–797 · doi:10.1097/00006250-199011000-00013 · PMID:2216226 · W2047559975
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Endometrial stromal cells cultured with peritoneal fluid from endometriosis patients exhibited significantly increased proliferation compared to those exposed to non-endometriosis peritoneal fluid.

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This in vitro study investigated whether factors present in the peritoneal fluid of patients with endometriosis influence the proliferation of endometrial stromal cells. Researchers cultured late proliferative phase endometrial stromal cells and exposed them to varying concentrations of cell-free peritoneal fluid obtained from women with and without endometriosis, matching samples by estradiol levels. The results demonstrated that stromal cell proliferation was significantly greater when exposed to fluid from endometriosis patients compared to control fluid or medium alone, exhibiting a linear dose-response relationship. These findings suggest that specific factors secreted into the peritoneal cavity of affected individuals may contribute to the growth or maintenance of endometriotic implants. This paper is centrally about endometriosis — specifically examining the paracrine effects of peritoneal fluid on endometrial stromal cell proliferation as a mechanism for disease progression.

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Abstract

Late proliferative phase endometrial stromal cells grown in short-term culture were used as a model for the stromal component of endometriotic implants. Cells were grown in medium alone and in medium supplemented by 5, 10, and 20% concentrations of the cell-free fractions of peritoneal fluid obtained from patients with and without endometriosis. Nine fluid-sample pairs were matched based on the presence or absence of endometriosis at laparoscopy and the similarity of peritoneal fluid estradiol concentrations. Stromal cell proliferation as reflected by 3H-thymidine incorporation during sequential cell harvests over 72 hours was greater for cells exposed to endometriosis peritoneal fluid than for those exposed to non-endometriosis peritoneal fluid. This reached statistical significance with exposure to peritoneal fluid concentrations of 10 and 20% (P less than .05). A linear dose-response relationship between 3H-thymidine incorporation and peritoneal fluid concentration could be derived only for stromal cells exposed to fluid samples obtained from endometriosis patients (r = 0.51; P less than .001). In addition, proliferation over 72 hours was significantly greater for cells grown in 20% endometriosis peritoneal fluid than for those grown in nutrient medium alone (P less than .001). These data imply that factor(s) secreted into the peritoneal fluid of endometriosis patients may play a role in the proliferation or maintenance of disease implants.
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Effect of Peritoneal Fluid From Endometriosis Patients on Endometrial Stromal Cell Proliferation In Vitro - ERIC S. SURREY - JOUKO HALME Late proliferative phase endometrial stromal cells grown in short-term culture were used as a model for the stromal component of endometriotic implants. Cells were grown in medium alone and in medium supplemented by 5, 10, and 20% concentrations of the cell-free fractions of peritoneal fluid obtained from patients with and without endometriosis. Nine fluid-sample pairs were matched based on the presence or absence of endometriosis at laparoscopy and the similarity of peritoneal fluid estradiol concentrations. Stromal cell proliferation as reflected by 3H-thymidine incorporation during sequential cell harvests over 72 hours was greater for cells exposed to endometriosis peritoneal fluid than for those exposed to non-endometriosis peritoneal fluid. This reached statistical significance with exposure to peritoneal fluid concentrations of 10 and 20% (P< .05). A linear dose-response relationship between 3H-thymidine incorporation and peritoneal fluid concentration could be derived only for stromal cells exposed to fluid samples obtained from endometriosis patients (r = 0.51;P< .001). In addition, proliferation over 72 hours was significantly greater for cells grown in 20% endometriosis peritoneal fluid than for those grown in nutrient medium alone (P< .001). These data imply that factor(s) secreted into the peritoneal fluid of endometriosis patients may play a role in the proliferation or maintenance of disease implants.(Obstet Gynecol 76:792, 1990)

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Condition tags

endometriosis

MeSH descriptors

Ascitic Fluid Endometriosis Endometrium Uterine Neoplasms Adult Cell Division Cell-Free System Cells, Cultured Culture Media Endometriosis Endometrium Female Humans In Vitro Techniques Menstrual Cycle Time Factors Uterine Neoplasms

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

Cited by (50)

SciLite annotations

chemicals 3
estradiol thymidine thymidine

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