Osteopontin Regulates Endometrial Stromal Cell Migration in Endometriosis through the PI3K Pathway

Xiaoxia Fu, Mengyun Yao, Chaoshuang Ye, Tao Fang, Ruijin Wu
Reproductive sciences (Thousand Oaks, Calif.) · 2020 · vol. 28(2) , pp. 435–446 · doi:10.1007/s43032-020-00301-8 · PMID:32909189 · W3085177036
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Abstract

Endometriosis is generally characterized as a tumor-like disease because of its potential for distant metastasis and local tissue invasion, while whether osteopontin (OPN) plays a role in the pathogenesis of endometriosis has not been thoroughly investigated. We investigated the expression of OPN, urokinase plasminogen activator (uPA), phosphatidylinositol 3 kinase (PI3K), and phospho-PI3 kinase (p-PI3K) in endometrial stromal cells (ESCs). The serum concentration of OPN was determined by enzyme-linked immunosorbent assays (ELISA). OPN was downregulated to explore the corresponding change of uPA, p-PI3K, F-actin, and α-tubulin. The expression of OPN, uPA, PI3K, and p-PI3K was evaluated by western blot and quantitative real-time PCR (RT-qPCR) and the expression of F-actin and α-tubulin was confirmed by immunofluorescence assay. The proliferation and migration abilities of ESCs were investigated by CCK8, transwell, and wound scratch assays. Endometrial OPN, p-PI3K, and uPA expressions and serum OPN levels were increased in patients with endometriosis compared with the control. The expressions of p-PI3K, uPA, and α-tubulin were decreased by siRNA-OPN interference in ectopic ESCs. Activation and inhibition of the PI3K pathway apparently upregulate and downregulate uPA expression. Knockdown of OPN and inhibition of the PI3K pathway remarkably inhibited cell migration in ectopic ESCs. Meanwhile, activation of the PI3K pathway promoted the migration ability of ectopic ESCs. OPN may regulate the expression of uPA through the PI3K signal pathway to affect the migration ability of ESCs, indicating that OPN, uPA, and the PI3K pathway may be potential targets for interrupting development of endometriosis.

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mesh:D004715endometriosis

MeSH descriptors

Cell Movement Endometriosis Endometrium Osteopontin Phosphatidylinositol 3-Kinase Stromal Cells Adult Case-Control Studies Cell Proliferation Cells, Cultured Endometriosis Endometriosis Endometriosis Endometrium Endometrium Female Humans Membrane Proteins Membrane Proteins Membrane Proteins

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