Effects of peritoneal macrophages from women with endometriosis on endometrial cellular proliferation in an in vitro coculture model

Loh FH, Foo-Hoe Loh, F H Loh, A Bongso, Ariff Bongso, Bongso A, C Y Fong, Chui‐Yee Fong, Fong CY, D R Koh, Dow-Rhoon Koh, Koh DR, Lee SH, S H Lee, Szu‐Hee Lee, Zhao HQ, H Q Zhao, Hui-Qin Zhao
Fertility and Sterility · 1999 · vol. 72(3) , pp. 533–538 · doi:10.1016/s0015-0282(99)00292-7 · PMID:10519630 · W2014647766
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This study investigated how peritoneal macrophages from women with endometriosis influence endometrial cell proliferation in an in vitro coculture model.

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Abstract

ObjectiveTo study the effects of peritoneal macrophages on endometrial cellular proliferation in an in vitro coculture model and to compare the magnitude of these effects between macrophages from women with endometriosis and normal women.DesignControlled study of peritoneal macrophage function.SettingUniversity hospital.Patient(s)Patients with a normal peritoneal cavity (n = 15) and with pelvic endometriosis (n = 20) undergoing laparoscopy.Intervention(s)Peritoneal macrophages were cocultured with endometrial epithelial and stromal cells; endometrial cell cultures without macrophage coculture acted as controls.Main outcome measure(s)Endometrial cellular proliferation measured by 3H-thymidine incorporation.Result(s)Endometrial epithelial cells cocultured with peritoneal macrophages from women with endometriosis showed significantly increased proliferation compared with cocultures using macrophages from normal women when assessed at 24 hours (1.56 versus 1.03 times, respectively, over control) and at 72 hours (1.55 versus 1.10 times over control). Endometrial stromal cells cocultured with peritoneal macrophages from women with endometriosis similarly exhibited increased proliferation compared with cocultures using macrophages from normal women when assessed at 24 hours (1.65 versus 1.17 times over control) and at 72 hours (1.65 versus 1.21 times over control).Conclusion(s)Peritoneal macrophages of patients with endometriosis stimulate cellular proliferation of endometrial epithelial and stromal cells in vitro.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Endometrium Macrophages, Peritoneal Adult Ascitic Fluid Ascitic Fluid Ascitic Fluid Cell Division Coculture Techniques Endometriosis Endometrium Epithelial Cells Epithelial Cells Female Humans Macrophages, Peritoneal Stromal Cells Stromal Cells

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Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

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