In‑vitro models of human endometriosis (Review)

Hongjie Fan

doi:10.3892/etm.2019.8363

In‑vitro models of human endometriosis (Review)

Hongjie Fan

Experimental and therapeutic medicine · 2019 · vol. 19(3) , pp. 1617–1625 · doi:10.3892/etm.2019.8363 · PMID:32104212 · PMC7027135 · W2994989617

review OA: gold CC0 ⤵ 17 in-corpus citations

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This review summarizes diverse in-vitro models utilizing various cell and tissue types to investigate endometriosis pathology, mechanisms, and potential therapeutic approaches.

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Abstract

Endometriosis is one of the most common benign gynecological diseases in women of reproductive age worldwide. In past decades, a number of in‑vitro models have been used to investigate the pathology and therapeutic methods for the treatment of endometriosis. The current review summarized the majority of currently available in‑vitro models, which utilize a variety of cell or tissues types, including endometriotic cell lines, primary endometrial stromal cells, endometrial stem cells, endometrial explants, peritoneal explants and immune cells. These cells or tissues are cultured individually, co‑cultured in 2D or 3D systems with various matrices or cultured in chicken chorioallantotic membranes and amniotic membranes culture systems. These models are able to represent one or more aspects of the process of endometriosis. These models are helpful and can be used to investigate the development of endometriosis and the underlying mechanisms of this disorder in detail, and help investigators select appropriate models for their experiments. Recently, the new concept of endometriosis as a fibrotic condition will lead research to investigate the differentiation of myofibroblasts and the development of fibrosis in endometriotic lesions, which will increase the development of novel models that can be used to investigate endometriotic fibrosis.

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endometriosis

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References (74)

Cited by (17)

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[1] Abnormally located SSEA1+/SOX9+ endometrial epithelial cells with a basalis-like phenotype in the eutopic functionalis layer may play a role in the pathogenesis of endometriosis via openalex

[2] Adhesion of human endometrial fragments to peritoneum in vitro via openalex

[3] Adhesion of human endometrium to the epithelial lining and extracellular matrix of amnion in vitro: an electron microscopic study via openalex

[4] Adhesion of shed menstrual tissue in an in-vitro model using amnion and peritoneum: a light and electron microscopic study via openalex

[5] Altered Immunity in Endometriosis: What Came First? via openalex

[6] Angiogenic properties of endometrial mesenchymal stromal cells in endothelial co-culture: an in vitro model of endometriosis via openalex

[7] Angiostatic agents prevent the development of endometriosis-like lesions in the chicken chorioallantoic membrane via openalex

[8] An <i>In Vitro</i> Model to Study the Pathogenesis of the Early Endometriosis Lesion via openalex

[9] A novel in vitro experimental model for ovarian endometriosis: the three-dimensional culture of human ovarian surface epithelial cells in collagen gels via openalex

[10] Antifibrotic properties of epigallocatechin-3-gallate in endometriosis via openalex

[11] Characteristics of Multipotent Mesenchymal Stromal Cells Isolated from Human Endometrium and Endometriosis Lesions via openalex

[12] Characteristics of Multipotent Mesenchymal Stromal Cells Isolated from the Endometrium and Endometriosis Lesions of Women with Malformations of the Internal Reproductive Organs via openalex

[13] Co-cultured endometrial stromal cells and peritoneal mesothelial cells for an in vitro model of endometriosis via openalex

[14] Combination of estrogen and dioxin is involved in the pathogenesis of endometriosis by promoting chemokine secretion and invasion of endometrial stromal cells via openalex

[15] Co-operation between the AKT and ERK signaling pathways may support growth of deep endometriosis in a fibrotic microenvironment in vitro† via openalex

[16] Daidzein-rich isoflavone aglycones inhibit cell growth and inflammation in endometriosis via openalex

[17] Diagnosis and management of endometriosis: summary of NICE guidance via openalex

[18] Effect of a dienogest for an experimental three-dimensional endometrial culture model for endometriosis via openalex

[19] Effect of interleukin-8 (IL-8), anti-IL-8, and IL-12 on endometrial cell survival in combined endometrial gland and stromal cell cultures derived from women with and without endometriosis via openalex

[20] Effect of platelet-derived growth factor on endometrial stromal cell proliferation in vitro: a model for endometriosis? via openalex

[21] Effects of peritoneal macrophages from women with endometriosis on endometrial cellular proliferation in an in vitro coculture model via openalex

[22] Endometrial cell adhesion in an in vitro model using intact amniotic membranes via openalex

[23] Expression of Glycodelin and Cyclooxygenase‐2 in Human Endometrial Tissue Following Three‐dimensional Culture via openalex

[24] Gene expression profiles and functional characterization of human immortalized endometriotic epithelial and stromal cells via openalex

[25] Imatinib decreases endometrial stromal cell transmesothial migration and proliferation in the extracellular matrix of modeled peritoneum via openalex

[26] Interleukin-1β induces cyclooxygenase-2 expression and promotes the invasive ability of human mesenchymal stem cells derived from ovarian endometrioma via openalex

[27] Interleukin-33 modulates inflammation in endometriosis via openalex

[28] In Vitro and In Vivo Effects of Tumor Suppressor Gene PTEN on Endometriosis: An Experimental Study via openalex

[29] In vitro culture of endometrial stromal and gland cells as a model for endometriosis: The effect of peritoneal fluid on proliferation via openalex

[30] In vitro model of stromal and epithelial immortalized endometriotic cells via openalex

[31] Involvement of the Wnt/β-Catenin Signaling Pathway in the Cellular and Molecular Mechanisms of Fibrosis in Endometriosis via openalex

[32] Isolation, characterization, and comparison of human endometrial and endometriosis cells in vitro via openalex

[33] Isolation, characterization, and comparison of human endometrial and endometriosis cells in vitro. via openalex

[34] LIM Kinase 1 Mediates Estradiol Effects on the Phosphorylation of Cofilinl in Eutopic Endometrial Stromal Cells During the Invasion and Proliferation of Endometriosis via openalex

[35] miR-200c suppresses endometriosis by targeting MALAT1 in vitro and in vivo via openalex

[36] Modeling the early endometriotic lesion: mesothelium-endometrial cell co-culture increases endometrial invasion and alters mesothelial and endometrial gene transcription via openalex

[37] Models of endometriosis and their utility in studying progression to ovarian clear cell carcinoma via openalex

[38] Oral contraceptives prevent the development of endometriosis in the chicken chorioallantoic membrane model via openalex

[39] Peritoneal Fluid Reduces Angiogenesis-Related MicroRNA Expression in Cell Cultures of Endometrial and Endometriotic Tissues from Women with Endometriosis via openalex

[40] Pharmacological blockage of the CXCR4-CXCL12 axis in endometriosis leads to contrasting effects in proliferation, migration, and invasion† via openalex

[41] Platelets drive smooth muscle metaplasia and fibrogenesis in endometriosis through epithelial–mesenchymal transition and fibroblast-to-myofibroblast transdifferentiation via openalex

[42] Possible Role of Phthalate in the Pathogenesis of Endometriosis: In Vitro, Animal, and Human Data via openalex

[43] Retrograde menstruation in healthy women and in patients with endometriosis. via openalex

[44] REVIEW ARTICLE: Three‐Dimensional Culture of Endometrial Cells: An <i>In Vitro</i> Model of Endometriosis via openalex

[45] Rodent Models of Experimental Endometriosis: Identifying Mechanisms of Disease and Therapeutic Targets via openalex

[46] Soft matrices inhibit cell proliferation and inactivate the fibrotic phenotype of deep endometriotic stromal cells<i>in vitro</i> via openalex

[47] Src as a novel therapeutic target for endometriosis via openalex

[48] The Origin and Pathogenesis of Endometriosis via openalex

[49] Three-dimensional in vitro culture of endometrial explants mimics the early stages of endometriosis via openalex

[50] Time to redefine endometriosis including its pro-fibrotic nature via openalex

[51] Tissue integrity is essential for ectopic implantation of human endometrium in the chicken chorioallantoic membrane via openalex

[52] Tumour necrosis factor-α, interleukin-6 and interleukin-8 do not promote adhesion of human endometrial epithelial cells to mesothelial cells in a quantitative in vitro model* via openalex

[53] Whole Endometrial Fragments Form Characteristics of In Vivo Endometriosis in a Mesothelial Cell Co-Culture System: An In Vitro Model for the Study of the Histogenesis of Endometriosis via openalex

[54] Whole explants of peritoneum and endometrium: a novel model of the early endometriosis lesion via openalex

[55] W6767670412 via openalex

[56] W2132577372 via openalex

[57] W1879876214 via openalex

[58] W1964923339 via openalex

[59] W1978573040 via openalex

[60] W1981263394 via openalex

[61] W1987520465 via openalex

[62] W1991381524 via openalex

[63] W2060449870 via openalex

[64] W2063058031 via openalex

[65] W2108787980 via openalex

[66] W2126728673 via openalex

[67] W2163315477 via openalex

[68] W2345264672 via openalex

[69] W2588408456 via openalex

[70] W2801672613 via openalex

[71] W6628680024 via openalex

[72] W6733003047 via openalex

[73] W6750853143 via openalex

[74] W2129503523 via openalex