Expression of Glycodelin and Cyclooxygenase‐2 in Human Endometrial Tissue Following Three‐dimensional Culture

Navid Esfandiari, Jafar Ai, Nazemian Z, Zohreh Nazemian, Murid H Javed, Javed MH, Murid Javed, Lynda Gotlieb, Gotlieb L, Casper RF, Robert F. Casper
American journal of reproductive immunology (New York, N.Y. : 1989) · 2006 · vol. 57(1) , pp. 49–54 · doi:10.1111/j.1600-0897.2006.00445.x · PMID:17156191 · W1997974115
article OA: closed CC0 ⤵ 17 in-corpus citations
View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-06-07 ⓘ

Three-dimensional culture of human endometrial tissue in a fibrin matrix promoted angiogenesis and cell proliferation, with glycodelin expressed in glands and COX-2 in new vessels after four weeks.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

PROBLEM: Our previous study showed that in vitro culture of human endometrial tissue in a three-dimensional (3D) fibrin matrix could mimic the early stages of endometriosis with invasion, gland and stroma formation and sprouting of new vessels. The objective of the present study was to evaluate the expression of glycodelin (Gd) and cyclooxygenase-2 (COX-2), two angiogenic factors, to further validate the 3D culture model of endometriosis. METHOD OF STUDY: Human endometrial fragments were obtained from endometrial biopsies and placed in a 3D fibrin matrix culture. Immunohistochemistry with specific antibodies to Gd and COX-2 was used to examine endometrial epithelium and blood vessels, and 4, 6-diamidino-2-phenylindole staining was used for nuclear identification. RESULTS: Three-dimensional culture of human endometrial tissue in the fibrin matrix resulted in the proliferation of endometrial stromal cells, glandular epithelium and angiogenesis. Gd positive glandular epithelium was seen in 85% of wells with developing endometrial glands and COX-2 positive new vessels were seen in 80% of wells with angiogenesis-like structures after 4 weeks of culture. CONCLUSION: Our findings confirm that angiogenesis occurs following the culture of endometrial tissue in the 3D fibrin matrix, and suggests that Gd and COX-2 might play important roles in promoting neovascularization and cell proliferation in the establishment of endometriosis.

My notes (saved in your browser only)

Condition tags

endometriosis

MeSH descriptors

Cyclooxygenase 2 Endometrium Glycoproteins Membrane Proteins Pregnancy Proteins Adult Cell Proliferation Cyclooxygenase 2 Endometrium Endometrium Female Glycodelin Glycoproteins Humans Membrane Proteins Pregnancy Proteins Tissue Culture Techniques

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (20)

Cited by (17)

Source provenance

europepmc
last seen: 2026-09-27T09:11:36.575535+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
pubmed
last seen: 2026-05-13T22:15:06.633332+00:00
unpaywall
last seen: 2026-09-28T06:20:55.906821+00:00
License: CC0 · commercial use OK