Endometrial cell adhesion in an in vitro model using intact amniotic membranes
article
OA: closed
CC0
⤵ 50 in-corpus citations
AI-generated summary
This study investigated endometrial cell adhesion to intact amniotic membranes using an in vitro model to explore potential mechanisms of endometriosis development.
One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works
Abstract
ObjectiveTo study adhesion between endometrial tissue and peritoneum using intact amniotic membranes as an in vitro model.DesignA cell biologic and immunohistochemical study.SettingTertiary-care university medical center.PatientsTen patients with regular and ovulatory cycles.InterventionsMechanically dissected tissue fragments from endometrial biopsies were cultured on either side of intact amniotic membranes. Also, the carcinoma cell lines RL95-2 and AN3CA were used.Main outcome measuresThe adhesion of endometrial fragments and of endometrial carcinoma cell lines to amniotic membrane was studied in vitro and evaluated using frozen sections. The composition of the extracellular matrix and the presence of intermediate filament proteins of amniotic membrane were determined using immunohistochemistry and compared with those of normal peritoneum.ResultsPeritoneum and amniotic membrane were similar with respect to expression of cytokeratins in epithelial lining and of extracellular matrix (ECM) components. The endometrial fragments did not adhere to the intact epithelial side of the amniotic membrane. In contrast, adhesion did occur to the nonepithelial side of the amnion. The carcinoma cell lines RL95-2 and AN3CA adhered to either side of intact amniotic membranes.ConclusionsAn intact epithelial lining prevents adhesion of endometrial fragments to the ECM of amniotic membranes in vitro. An intact epithelium could be important defense mechanism in preventing initial adhesion of retrogradely shed endometrium fragments to peritoneum.
My notes (saved in your browser only)
Citation neighborhood
Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.
References (17)
- 5 Appearances of endometriosis via openalex
- Endometriosis and mullerian anomalies. via openalex
- Expression of integrins E-cadherin in cells from menstrual effluent, endometrium, peritoneal fluid, peritoneum, endometriosis via openalex
- Functional aspects of peritoneal macrophages in endometriosis of women via openalex
- P-Cadherin Expression in Human Endometrium and Endometriosis via openalex
- The development of the implantation theory for the origin of peritoneal endometriosis via openalex
- Whole Endometrial Fragments Form Characteristics of In Vivo Endometriosis in a Mesothelial Cell Co-Culture System: An In Vitro Model for the Study of the Histogenesis of Endometriosis via openalex
- doi:10.1016/0002-9378(60)90512-3 via openalex
- doi:10.1016/s0021-9258(17)44226-8 via openalex
- doi:10.3109/01913128509141519 via openalex
- doi:10.1016/0304-3835(80)90105-6 via openalex
- doi:10.1016/s0022-5320(65)80089-2 via openalex
- doi:10.1016/0002-9378(58)90610-0 via openalex
- W1481997113 via openalex
- W6702803669 via openalex
- doi:10.1007/bf00533396 via openalex
- doi:10.1016/s0025-6196(12)62290-3 via openalex
Cited by (50)
- Stilbene-based strategies for the management of endometriosis: targeting cell adhesion, migration, and ECM-driven invasion 2026
- Etiologies of endometriosis and model systems: is there a risk of a tunnel vision? 2025
- In‑vitro models of human endometriosis (Review) 2019
- Endometriosis 2017
- Laparoscopic Surgery: A New Technique to Induce Endometriosis in a Mouse Model 2016
- Genes Linked to Endometriosis by GWAS Are Integral to Cytoskeleton Regulation and Suggests That Mesothelial Barrier Homeostasis Is a Factor in the Pathogenesis of Endometriosis 2016
- Endometriosis ciática: una causa poco conocida de ciatalgia 2013
- Models of Endometriosis:<i>In vitro</i>and<i>In vivo</i>Models 2011
- Peritoneal, Ovarian, and Rectovaginal Endometriosis are Three Different Entities 2011
- Tumor Necrosis Factor-α and Interleukin-1β Treatment of Endometrial Stromal Cells Does not Promote Their Adhesion to Peritoneal Mesothelial Cells in an in Vitro Model of Early-Stage Endometriosis 2011
- Evolving Spectrum: 2010
- In Vitro Models to Study the Pathogenesis of Endometriosis 2009
- Modeling the early endometriotic lesion: mesothelium-endometrial cell co-culture increases endometrial invasion and alters mesothelial and endometrial gene transcription 2008
- Pathogenesis of endometriosis: The role of defective ‘immunosurveillance’ 2007
- Paracrine regulation of endometriotic tissue 2007
- [Endometriosis: physiopathology and investigation trends (first part)]. 2007
- Tumour necrosis factor-α, interleukin-6 and interleukin-8 do not promote adhesion of human endometrial epithelial cells to mesothelial cells in a quantitative in vitro model* 2005
- The pathogenesis of endometriosis: the endometrium - mesothelium dialogue 2004
- Pathogenesis of endometriosis — Current research 2003
- Pathogenesis of endometriosis 2003
- Three-dimensional in vitro culture of endometrial explants mimics the early stages of endometriosis 2003
- Pathogenesis of Endometriosis 2003
- Culture of menstrual endometrium with peritoneal explants and mesothelial monolayers confirms attachment to intact mesothelial cells 2002
- Enhanced T cells interactions with extracellular matrix proteins in infertile women with endometriosis 2002
- An <i>In Vitro</i> Model to Study the Pathogenesis of the Early Endometriosis Lesion 2002
- Immunology of endometriosis 2002
- Endometriosis, retrograde menstruation and peritoneal inflammation in women and in baboons 2002
- Peritoneal defects and the development of endometriosis in relation to the timing of endoscopic surgery during the menstrual cycle 2002
- In-vitro adhesion of endometrium to autologous peritoneal membranes: effect of the cycle phase and the stage of endometriosis 2002
- Pathogenesis of Endometriosis 2002
- Theories of endometriosis 2001
- Immunobiology of endometriosis 2001
- High ω-3:ω-6 fatty acid ratios in culture medium reduce endometrial-cell survival in combined endometrial gland and stromal cell cultures from women with and without endometriosis 2001
- Attachment of endometrial cells to peritoneum—facilitated? Reply of the authors: 2001
- Short-term culture of peritoneum explants confirms attachment of endometrium to intact peritoneal mesothelium 2001
- 10.1016/s1047-9422(03)00073-7 2000
- Early-stage endometriosis: adhesion and growth of human menstrual endometrium in nude mice 2000
- Transplantation of cultured explants of human endometrium into nude mice 2000
- Adhesion of shed menstrual tissue in an in-vitro model using amnion and peritoneum: a light and electron microscopic study 1999
- Current Concepts in the Pathogenesis of Endometriosis 1999
- Whole explants of peritoneum and endometrium: a novel model of the early endometriosis lesion 1999
- Adhesion of human endometrial fragments to peritoneum in vitro 1999
- Angiogenesis and Macrophage Activation in Endometriosis 1997
- A very sticky riddle 1997
- Reply of the authors 1997
- ENDOMETRIOSIS: A REVIEW OF ITS PATHOGENESIS 1997
- Clinical relevance of the baboon as a model for the study of endometriosis 1997
- PATHOGENESIS OF ENDOMETRIOSIS 1997
- Peritoneal endometriosis, ovarian endometriosis, and adenomyotic nodules of the rectovaginal septum are three different entities 1997
- Endometriosis a review of its pathogenesis 1997
Source provenance
- openalex
- last seen: 2026-05-14T06:16:54.145842+00:00
License: CC0
· commercial use OK