High ω-3:ω-6 fatty acid ratios in culture medium reduce endometrial-cell survival in combined endometrial gland and stromal cell cultures from women with and without endometriosis

M.Rafet Gazvani, M R Gazvani, Gazvani MR, Smith L, Louise Smith, L Smith, Paul Haggarty, Haggarty P, P Haggarty, Paul Fowler, P. A. Fowler, Templeton A, A Templeton, Allan Templeton
Fertility and sterility · 2001 · vol. 76(4) , pp. 717–722 · doi:10.1016/s0015-0282(01)01991-4 · PMID:11591404 · W2026760806
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This study investigated the impact of varying omega-3 to omega-6 fatty acid ratios in culture medium on endometrial cell survival, finding that higher ratios reduced survival in both endometriosis and control endometrial gland and stromal cell cultures.

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Abstract

ObjectiveTo study the effects of omega-3 and omega-6 polyunsaturated fatty acid (PUFA) on in vitro proliferation of endometrial cells and their production of the cytokine interleukin-8 (IL-8).DesignIn vitro study.SettingObstetrics and gynecology department, University of Aberdeen.Patient(s)Women attending an infertility clinic.Intervention(s)In vitro cell cultures using culture mediums supplemented with normal and high ratios of omega-3 PUFA and omega-6 PUFA.Main outcome measure(s)In vitro survival and production of IL-8 by dispersed endometrial cells.Result(s)In vitro survival of endometrial cells from women with and without endometriosis was significantly reduced in the presence of high omega-3:omega-6 PUFA ratios compared with cells incubated in the absence of fatty acids, in balanced omega-3:omega-6 PUFA ratios, and in high omega-6:omega-3 PUFA ratios. Endometrial cells from women with endometriosis secreted higher concentrations of IL-8, especially in the presence of high omega-3:omega-6 PUFA ratios.Conclusion(s)omega-3 PUFA may have a suppressive effect on the in vitro survival of endometrial cells and omega-3 PUFA be useful in the management of endometriosis by reducing the inflammatory response and modulating cytokine function.

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Condition tags

endometriosis

MeSH descriptors

Culture Media Endometriosis Endometrium Fatty Acids, Omega-3 Fatty Acids, Unsaturated Stromal Cells Cells, Cultured Cell Survival Cell Survival Culture Media Endometriosis Endometriosis Endometrium Endometrium Fatty Acids, Omega-3 Fatty Acids, Omega-6 Fatty Acids, Unsaturated Female Humans Interleukin-8

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