Whole Endometrial Fragments Form Characteristics of In Vivo Endometriosis in a Mesothelial Cell Co-Culture System: An In Vitro Model for the Study of the Histogenesis of Endometriosis
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This study developed an in vitro mesothelial cell co-culture system that demonstrated whole endometrial fragments forming characteristics of in vivo endometriosis.
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Abstract
ObjectiveWe have previously reported on the effects of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 (IL-1) on the adhesion of human endometrial stromal cells to peritoneal mesothelial cells in vitro. The relevance of this co-culture system to in vivo endometriosis remains to be established. We contrasted endometrial fragments with endometrial stromal cells to determine their relevance.MethodsHuman mesothelial cells were isolated from peritoneal fluid from four normal women via Ficoll-Paque gradient centrifugation at 400 x g for 30 minutes and grown in M199 medium containing epidermal growth factor (10 ng/mL) and 10% fetal calf serum. The homogeneous cells were cultured on collagen-coated plates until a monolayer formed. Endometrial stromal cells or whole endometrial fragments, isolated from endometrial tissue of four normal women, were put on the mesothelial monolayer and cultured at 37 degrees C for 24 days. After fixation, the samples were incubated with monoclonal antibody to epithelial membrane antigen, cytokeratin, and vimentin, and processed with standard immunohistochemical techniques.ResultsObservation under phase contrast microscopy revealed that, in this co-culture system, whole endometrial fragments demonstrated characteristic morphology of in vivo endometriosis, whereas isolated endometrial stromal cells did not.ConclusionWhole endometrial fragments, but not isolated endometrial stromal cells, form morphologically characteristic structures similar to in vivo endometriosis in this co-culture system. It is hoped that this system might be useful for studying certain aspects of the histogenesis of endometriosis.
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- Peritoneal endometriosis due to the menstrual dissemination of endometrial tissue into the peritoneal cavity via openalex
- W1971321449 via openalex
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Cited by (17)
- Activity Methods for Endometriosis 2023
- In‑vitro models of human endometriosis (Review) 2019
- Identifying the Biological Basis of GWAS Hits for Endometriosis1 2015
- Attachment to extracellular matrices is enhanced in human endometriotic stromal cells: a possible mechanism underlying the pathogenesis of endometriosis 2010
- In Vitro Models to Study the Pathogenesis of Endometriosis 2009
- Endometrial paradigms 2008
- Tumour necrosis factor-α, interleukin-6 and interleukin-8 do not promote adhesion of human endometrial epithelial cells to mesothelial cells in a quantitative in vitro model* 2005
- Adhesion of endometrial cells labeled with 111Indium-tropolonate to peritoneum: a novel in vitro model to study endometriosis 2003
- An <i>In Vitro</i> Model to Study the Pathogenesis of the Early Endometriosis Lesion 2002
- Endometriosis, retrograde menstruation and peritoneal inflammation in women and in baboons 2002
- In-vitro adhesion of endometrium to autologous peritoneal membranes: effect of the cycle phase and the stage of endometriosis 2002
- Morphological changes in mesothelial cells induced by shed menstrual endometrium in vitro are not primarily due to apoptosis or necrosis 2000
- Expression of the α2β1 and α3β1 integrins at the surface of mesothelial cells: a potential attachment site of endometrial cells 2000
- Whole explants of peritoneum and endometrium: a novel model of the early endometriosis lesion 1999
- Adhesion of shed menstrual tissue in an in-vitro model using amnion and peritoneum: a light and electron microscopic study 1999
- Fibrinolytic factors in endometriotic tissue, endometrium, peritoneal fluid, and plasma from women with endometriosis and in endometrium and peritoneal fluid from healthy women 1998
- Endometrial cell adhesion in an in vitro model using intact amniotic membranes 1996
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- europepmc
- last seen: 2026-07-28T06:14:09.330459+00:00
- openalex
- last seen: 2026-06-10T17:14:06.276822+00:00
- pubmed
- last seen: 2026-05-13T22:11:29.222973+00:00
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