Endometrial cell adhesion in an in vitro model using intact amniotic membranes
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This study investigated endometrial cell adhesion to intact amniotic membranes using an in vitro model to explore potential mechanisms of endometriosis development.
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Abstract
OBJECTIVE: To study adhesion between endometrial tissue and peritoneum using intact amniotic membranes as an in vitro model.
DESIGN: A cell biologic and immunohistochemical study.
SETTING: Tertiary-care university medical center.
PATIENTS: Ten patients with regular and ovulatory cycles.
INTERVENTIONS: Mechanically dissected tissue fragments from endometrial biopsies were cultured on either side of intact amniotic membranes. Also, the carcinoma cell lines RL95-2 and AN3CA were used.
MAIN OUTCOME MEASURES: The adhesion of endometrial fragments and of endometrial carcinoma cell lines to amniotic membrane was studied in vitro and evaluated using frozen sections. The composition of the extracellular matrix and the presence of intermediate filament proteins of amniotic membrane were determined using immunohistochemistry and compared with those of normal peritoneum.
RESULTS: Peritoneum and amniotic membrane were similar with respect to expression of cytokeratins in epithelial lining and of extracellular matrix (ECM) components. The endometrial fragments did not adhere to the intact epithelial side of the amniotic membrane. In contrast, adhesion did occur to the nonepithelial side of the amnion. The carcinoma cell lines RL95-2 and AN3CA adhered to either side of intact amniotic membranes.
CONCLUSIONS: An intact epithelial lining prevents adhesion of endometrial fragments to the ECM of amniotic membranes in vitro. An intact epithelium could be important defense mechanism in preventing initial adhesion of retrogradely shed endometrium fragments to peritoneum.
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- Endometriosis 2017
- Genes Linked to Endometriosis by GWAS Are Integral to Cytoskeleton Regulation and Suggests That Mesothelial Barrier Homeostasis Is a Factor in the Pathogenesis of Endometriosis 2016
- Laparoscopic Surgery: A New Technique to Induce Endometriosis in a Mouse Model 2016
- Endometriosis ciática: una causa poco conocida de ciatalgia 2013
- Models of Endometriosis:<i>In vitro</i>and<i>In vivo</i>Models 2011
- Tumor Necrosis Factor-α and Interleukin-1β Treatment of Endometrial Stromal Cells Does not Promote Their Adhesion to Peritoneal Mesothelial Cells in an in Vitro Model of Early-Stage Endometriosis 2011
- Peritoneal, Ovarian, and Rectovaginal Endometriosis are Three Different Entities 2011
- Evolving spectrum: the pathogenesis of endometriosis 2010
- Reviews: in vitro models to study the pathogenesis of endometriosis 2009
- Modeling the early endometriotic lesion: mesothelium-endometrial cell co-culture increases endometrial invasion and alters mesothelial and endometrial gene transcription 2008
- Paracrine regulation of endometriotic tissue 2007
- Pathogenesis of endometriosis: the role of defective 'immunosurveillance' 2007
- [Endometriosis: physiopathology and investigation trends (first part)] 2007
- Tumour necrosis factor-alpha, interleukin-6 and interleukin-8 do not promote adhesion of human endometrial epithelial cells to mesothelial cells in a quantitative in vitro model 2005
- The pathogenesis of endometriosis: the endometrium - mesothelium dialogue 2004
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- Pathogenesis of endometriosis--current research 2003
- In-vitro adhesion of endometrium to autologous peritoneal membranes: effect of the cycle phase and the stage of endometriosis 2002
- Culture of menstrual endometrium with peritoneal explants and mesothelial monolayers confirms attachment to intact mesothelial cells 2002
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- Immunology of endometriosis 2002
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- Pathogenesis of endometriosis 2002
- Peritoneal defects and the development of endometriosis in relation to the timing of endoscopic surgery during the menstrual cycle 2002
- Endometriosis, retrograde menstruation and peritoneal inflammation in women and in baboons 2002
- Attachment of endometrial cells to peritoneum—facilitated? Reply of the authors: 2001
- High omega-3:omega-6 fatty acid ratios in culture medium reduce endometrial-cell survival in combined endometrial gland and stromal cell cultures from women with and without endometriosis 2001
- Theories of endometriosis 2001
- Immunobiology of endometriosis 2001
- Short-term culture of peritoneum explants confirms attachment of endometrium to intact peritoneal mesothelium 2001
- Transplantation of cultured explants of human endometrium into nude mice 2000
- Early-stage endometriosis: adhesion and growth of human menstrual endometrium in nude mice 2000
- Current concepts in the pathogenesis of endometriosis 1999
- Whole explants of peritoneum and endometrium: a novel model of the early endometriosis lesion 1999
- Adhesion of human endometrial fragments to peritoneum in vitro 1999
- Adhesion of shed menstrual tissue in an in-vitro model using amnion and peritoneum: a light and electron microscopic study 1999
- Endometriosis: a review on its pathogenesis 1997
- Clinical relevance of the baboon as a model for the study of endometriosis 1997
- Pathogenesis of endometriosis 1997
- ENDOMETRIOSIS: A REVIEW OF ITS PATHOGENESIS 1997
- A very sticky riddle 1997
- Peritoneal endometriosis, ovarian endometriosis, and adenomyotic nodules of the rectovaginal septum are three different entities 1997
- Angiogenesis and macrophage activation in endometriosis 1997
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