Isolation, characterization, and comparison of human endometrial and endometriosis cells in vitro.

I P Ryan, I. Ryan, E D Schriock, Eldon D. Schriock, R N Taylor, Robert N. Taylor
The Journal of clinical endocrinology and metabolism · 1994 · vol. 78(3) , pp. 642–649 · doi:10.1210/jcem.78.3.8126136 · PMID:8126136 · W2054565450
article OA: closed CC0 ⤵ 150 in-corpus citations
View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by claude@2026-06, 2026-06-07 ⓘ

This study isolated and characterized human endometrial and endometriosis epithelial and stromal cells in vitro, finding they shared phenotypic and functional markers including hormone responsiveness.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

Endometriosis is a common gynecological disorder of unclear pathogenesis. We have established an in vitro model to investigate phenotypic similarities and differences between normal endometrial and endometriosis cells. Highly purified cultures of epithelial and stromal cells were isolated from normal endometrium and endometriosis implants. Morphological features as well as immunocytochemical markers confirm these isolates as epithelial and stromal cells. Potential hormone responsiveness was established by the documentation of estrogen receptor mRNA in epithelial and stromal cells isolated from both tissue types. Expression of this receptor protein was verified in stromal cells by competitive radioligand binding, revealing comparable receptor numbers and dissociation constants. CA-125 is selectively secreted in similar concentrations by epithelial cells isolated from both tissue types. PRL secretion is selectively exhibited by progestin-stimulated stromal cells from both tissue types. Our findings demonstrate that highly purified epithelial and stromal cells cultured from normal endometrial and endometriosis tissues express the same phenotypic and functional markers as their in vivo counterparts. These cultures provide useful models to identify endometriosis-specific cell products that contribute to the pathogenesis of this disorder.

My notes (saved in your browser only)

Condition tags

mesh:D004715endometriosis

MeSH descriptors

Endometriosis Endometrium Adult Base Sequence Cells, Cultured Cell Separation Endometriosis Endometrium Endometrium Female Histocytochemistry Humans Immunohistochemistry Middle Aged Molecular Sequence Data Oligonucleotide Probes Oligonucleotide Probes Polymerase Chain Reaction Receptors, Estrogen Receptors, Estrogen

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (24)

Cited by (50)

Source provenance

europepmc
last seen: 2026-06-04T01:30:01.192114+00:00
openalex
last seen: 2026-06-04T00:00:01.174412+00:00
pubmed
last seen: 2026-05-13T22:11:29.222973+00:00
License: CC0 · commercial use OK