Isolation, characterization, and comparison of human endometrial and endometriosis cells in vitro

Ryan IP, I. Ryan, I. P. Ryan, Schriock ED, E D Schriock, Eldon D. Schriock, Taylor RN, R N Taylor, Robert N. Taylor
The Journal of clinical endocrinology and metabolism · 1994 · vol. 78(3) , pp. 642–649 · doi:10.1210/jcem.78.3.8126136 · PMID:8126136 · W2054565450
Lab / animal OA: closed CC0 ⤵ 150 in-corpus citations
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This study isolated and characterized human endometrial and endometriosis epithelial and stromal cells in vitro, finding they shared phenotypic and functional markers including hormone responsiveness.

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Abstract

Endometriosis is a common gynecological disorder of unclear pathogenesis. We have established an in vitro model to investigate phenotypic similarities and differences between normal endometrial and endometriosis cells. Highly purified cultures of epithelial and stromal cells were isolated from normal endometrium and endometriosis implants. Morphological features as well as immunocytochemical markers confirm these isolates as epithelial and stromal cells. Potential hormone responsiveness was established by the documentation of estrogen receptor mRNA in epithelial and stromal cells isolated from both tissue types. Expression of this receptor protein was verified in stromal cells by competitive radioligand binding, revealing comparable receptor numbers and dissociation constants. CA-125 is selectively secreted in similar concentrations by epithelial cells isolated from both tissue types. PRL secretion is selectively exhibited by progestin-stimulated stromal cells from both tissue types. Our findings demonstrate that highly purified epithelial and stromal cells cultured from normal endometrial and endometriosis tissues express the same phenotypic and functional markers as their in vivo counterparts. These cultures provide useful models to identify endometriosis-specific cell products that contribute to the pathogenesis of this disorder.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Endometrium Adult Adult Base Sequence Base Sequence Cells, Cultured Cells, Cultured Cell Separation Cell Separation Endometriosis Endometriosis Endometrium Endometrium Endometrium Female Female Histocytochemistry Histocytochemistry Humans

Citation neighborhood (2-hop)

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

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Cited by (50)

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