Co-cultured endometrial stromal cells and peritoneal mesothelial cells for an in vitro model of endometriosis

Zhenling Chen, Yi Dai, Zhe Dong, Menghui Li, Xuan Mu, Rui Zhang, Zhuo Wang, Wei Zhang, Jinghe Lang, Jinhua Leng, Xingyu Jiang
Integrative biology : quantitative biosciences from nano to macro · 2012 · vol. 4(9) , pp. 1090 · doi:10.1039/c2ib00172a · PMID:22772808 · W2010215885
article OA: closed CC0 ⤵ 11 in-corpus citations
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⚙ AI-generated summary by claude@2026-06, 2026-06-08 ⓘ

This study developed an in vitro model mimicking endometriosis by co-culturing endometrial and peritoneal mesothelial cells, finding that peritoneal mesothelial cells from endometriotic individuals failed to resist endometrial stromal cell invasion.

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Abstract

This paper demonstrates an in vitro model to simulate the microenvironment of endometriosis. We used microfluidic channels with cover slips to pattern and release endometrial stromal cells (ESCs) and human peritoneal mesothelial cells (HPMCs) in a way that mimicked the pathophysiology of peritoneal endometriosis. This approach enabled observation in real time interactions between ESCs and HPMCs both in their normal and pathological states. HPMCs from control individuals were able to resist the invasion of ESCs from both control and endometriotic individuals. By contrast, HPMCs from endometriotic individuals were unable to resist the invasion of ESCs from both normal and endometriotic individuals. We further analyzed the dynamics between HPMCs and ESCs from endometriotic individuals. HPMCs from endometriotic individuals relaxed their adhesion to each other at the beginning of invasion of ESCs, lose their adhesion to the substrate and apoptosed when surrounded by ESCs. These data implicate that the peritoneal physiology may play an important role in endometriosis.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Epithelial Cells Peritoneal Diseases Stromal Cells Adult Coculture Techniques Endometriosis Epithelial Cells Epithelial Cells Female Humans Microfluidics Microscopy, Fluorescence Microscopy, Phase-Contrast Middle Aged Peritoneal Diseases Stromal Cells Stromal Cells

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (22)

Cited by (11)

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