Release of tumor necrosis factor-α by human peritoneal macrophages in vivo and in vitro

Halme J, J Halme, Jouko Halme
American Journal of Obstetrics and Gynecology · 1989 · vol. 161(6) , pp. 1718–1725 · doi:10.1016/0002-9378(89)90957-5 · PMID:2603932 · W2050833253
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This study investigated the release of tumor necrosis factor-α by human peritoneal macrophages in both living organisms and laboratory settings.

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Abstract

Tumor necrosis factor is a product of activated monocytes, macrophages, and lymphocytes that exerts a variety of effects in the host. Its cytotoxicity toward gametes has been suggested as a mechanism of how activated macrophages may cause subfertility, inasmuch as detectable levels of tumor necrosis factor have been reported in the peritoneal fluid of infertile patients. To further examine this issue we measured tumor necrosis factor activity in peritoneal fluid and its release in vitro from monocytes or peritoneal macrophages of patients undergoing laparoscopy because of either tubal ligation or infertility. Cytolytic activity was determined with a bioassay with sensitized mouse fibrosarcoma cells as target. Significant tumor necrosis factor activity (greater than 5 U/ml) was detected in 38% of the peritoneal fluid samples. A total of 43% of the peritoneal macrophage samples released significant tumor necrosis factor activity in vitro and all samples tested including peripheral monocytes released high levels of activity in the presence of bacterial endotoxin. The activity was released in vitro in a time-dependent manner. Thermal stability characteristics and neutralization with a specific antibody indicated that most of the activity in peritoneal fluid or released in vitro was that of tumor necrosis factor-alpha. Statistical analysis of tumor necrosis factor activity in peritoneal fluid in various diagnostic categories revealed a significantly elevated level in patients with endometriosis, as compared with fertile women. Endometriosis and pelvic adhesions were also significantly more likely to be associated with measurable levels of tumor necrosis factor activity released by peritoneal macrophages in vitro. Overall, because tumor necrosis factor-alpha levels were frequently detectable, it suggests that activation of peritoneal macrophages either by bacterial products or inflammatory conditions can occur in vivo. Moreover, these cells remain competent to release tumor necrosis factor in vitro.

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MeSH descriptors

Macrophages Tumor Necrosis Factor-alpha Adult Ascitic Fluid Ascitic Fluid Cell Adhesion Cell Adhesion Cells, Cultured Female Humans Macrophage Activation Macrophage Activation Macrophages Macrophages Peritoneal Cavity Peritoneal Cavity Tumor Necrosis Factor-alpha Tumor Necrosis Factor-alpha

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