Endometriosis is associated with alterations in the relative abundance of proteins and IL-10 in the peritoneal fluid

Siamak Tabibzadeh, Jeanne L Becker, Anna K Parsons
Frontiers in bioscience : a journal and virtual library · 2003 · vol. 8(1) , pp. a70–78 · doi:10.2741/1045 · PMID:12700072 · W1991760399
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⚙ AI-generated summary by claude@2026-06, 2026-06-09 ⓘ

Endometriosis alters peritoneal fluid protein profiles and increases IL-10 levels, with peritoneal cells identified as a primary source of IL-10.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-09 · read from full text ⓘ

This study compared the protein profile of peritoneal fluids from normal fertile women, women with infertility without evidence of endometriosis, and women with mild to severe endometriosis using two-dimensional gel electrophoresis, complemented by ELISA for IL-10 and RT-PCR for IL-10 mRNA. Fertility controls showed a distinct reproducible protein pattern, while infertility without endometriosis did not alter relative protein abundance; mild endometriosis corresponded to mild reductions in specific protein spots, and severe endometriosis showed both more pronounced reductions and appearance of new protein spots not detected in normals. Moderate to severe endometriosis was associated with markedly elevated peritoneal IL-10 levels, with IL-10 mRNA detected in cells derived from peritoneal fluid. The paper does not explicitly state limitations in the provided text. This paper is centrally about endometriosis—specifically, how peritoneal protein abundance patterns and IL-10 levels differ across disease severity in endometriosis.

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Abstract

A growing body of evidence suggests that endometriosis modulates the microenvironment of peritoneal cavity. Therefore, in this study, we compared the protein profile of peritoneal fluids from normal fertile women with those from patients with infertility, and patients with mild to severe endometriosis. Two-dimensional gel electrophoresis of peritoneal fluids from normal subjects exhibited a distinct and reproducible pattern of proteins in the size ranges of approximately 35 to 80 kD and pI close to 4.5 to 6.6. Infertility without evidence of endometriosis was not associated with changes in the relative abundance of proteins present in the peritoneal fluid. However, mild endometriosis was associated with a mild reduction in the amount of several peritoneal protein spots with the approximate molecular weights of 35-40 kD and pI close to 5.7-6.0. These changes became markedly apparent in the peritoneal fluid of patients who suffered from the severe form of this disease. Severe endometriosis was also associated with appearance of protein spots in the gels that were not detectable in the peritoneal fluids of normal subjects. Consistent with these data, enzyme-linked immunosorbent assay showed that moderate to severe endometriosis was associated with markedly elevated levels of IL-10 in the peritoneal fluid. Reverse transcription followed by polymerase chain reaction amplification using primers specific to IL-10 confirmed presence of IL-10 mRNA in cells derived from peritoneal fluids. These findings show that endometriosis is associated with disturbed secretion of proteins into the peritoneal cavity and with an elevated level of IL-10 in the peritoneal fluid. The studies also show cells resident in peritoneum as a major source of IL-10.
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Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience. 1 Department of Obstetrics and Gynecology and Fetomaternal Medicine, Stony Brook University, Stony Brook, NY, USA 2 Department of Obstetrics and Gynecology, University of South Florida, Tampa, FL Abstract A growing body of evidence suggests that endometriosis modulates the microenvironment of peritoneal cavity. Therefore, in this study, we compared the protein profile of peritoneal fluids from normal fertile women with those from patients with infertility, and patients with mild to severe endometriosis. Two-dimensional gel electrophoresis of peritoneal fluids from normal subjects exhibited a distinct and reproducible pattern of proteins in the size ranges of approximately 35 to 80 kD and pI close to 4.5 to 6.6. Infertility without evidence of endometriosis was not associated with changes in the relative abundance of proteins present in the peritoneal fluid. However, mild endometriosis was associated with a mild reduction in the amount of several peritoneal protein spots with the approximate molecular weights of 35-40 kD and pI close to 5.7-6.0. These changes became markedly apparent in the peritoneal fluid of patients who suffered from the severe form of this disease. Severe endometriosis was also associated with appearance of protein spots in the gels that were not detectable in the peritoneal fluids of normal subjects. Consistent with these data, enzyme-linked immunosorbent assay showed that moderate to severe endometriosis was associated with markedly elevated levels of IL-10 in the peritoneal fluid. Reverse transcription followed by polymerase chain reaction amplification using primers specific to IL-10 confirmed presence of IL-10 mRNA in cells derived from peritoneal fluids. These findings show that endometriosis is associated with disturbed secretion of proteins into the peritoneal cavity and with an elevated level of IL-10 in the peritoneal fluid. The studies also show cells resident in peritoneum as a major source of IL-10.

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Condition tags

mesh:D004715endometriosisinfertility

MeSH descriptors

Ascitic Fluid Endometriosis Interleukin-10 Proteins Ascitic Fluid Ascitic Fluid Endometriosis Endometriosis Female Fertility Humans Infertility, Female Interleukin-10 Interleukin-10 Molecular Weight Protein Biosynthesis Proteins Proteins Proteins RNA, Messenger

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