Absence of a Direct Effect of Recombinant Interleukins and Cultured Peritoneal Macrophages on Early Embryonic Development in the Mouse1

Schneider EG, E G Schneider, Erwin Schneider, Armant DR, D R Armant, D. Randall Armant, T S Kupper, Kupper TS, Thomas S. Kupper, M L Polan, Mary Lake Polan, Polan ML
Biology of reproduction · 1989 · vol. 40(4) , pp. 825–833 · doi:10.1095/biolreprod40.4.825 · PMID:2787673 · W2121252968
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Recombinant interleukins (IL-1 and IL-2) and cultured human peritoneal macrophages did not impair in vitro early mouse embryonic development or implantation.

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Abstract

On the basis of their recent studies, several researchers have suggested that the infertility associated with mild endometriosis is due to the alteration of peritoneal fluid, resulting in impairment of the viability of gametes or embryos. Elevated numbers of macrophages and lymphocytes have been reported in the peritoneal fluid of patients with endometriosis. Interleukin-1 (IL-1) a major product of activated macrophages and Interleukin-2 (IL-2), a product of most activated T-cells, have been postulated to play a role in the infertility associated with this disease, possibly by acting as direct embryotoxic agents. We have examined the effect of purified recombinant IL-1 and IL-2, which are not species-specific, on in vitro development of mouse embryos. Both interleukins had no effect on development to the blastocyst stage or on early stages of implantation, as measured in vitro by attachment and outgrowth of blastocysts to fibronectin-coated dishes. Moreover, co-culture of mouse embryos with activated human peritoneal macrophages had no effect on embryogenesis. We conclude that neither IL-1, nor other products of human macrophages activated by lipopolysaccharide, nor IL-2 are directly toxic to early mouse embryonic development.

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Condition tags

endometriosisinfertility

MeSH descriptors

Embryonic and Fetal Development Interleukin-1 Macrophages Animals Blastocyst Blastocyst Cells, Cultured Embryo Implantation Endometriosis Endometriosis Endometriosis Female Humans Interleukin-1 Interleukin-2 Interleukin-2 Macrophages Mice Recombinant Proteins Recombinant Proteins

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