Endometriosis: Secretion of interleukin-6 by human endometriotic cells and regulation by proinflammatory cytokines and sex steroids
article
OA: closed
CC0
⤵ 86 in-corpus citations
Abstract
Endometriosis is generally associated with an immunoinflammatory process that takes place in the peritoneal cavity of patients. Interleukin (IL)-6, a multifunctional cytokine involved in numerous immunological and proliferative processes, has been found at high concentrations in the peritoneal fluid of endometriosis patients. The purpose of this study was to investigate the ability of endometriotic cells to produce IL-y and to assess the regulation of its secretion by proinflammatory cytokines and sex steroids. Cultures of human endometriotic cells were exposed to different concentrations of cytokines and sex steroid hormones for varying periods of time. IL-6 secretion was measured using an enzyme-linked immunosorbent assay. Endometriotic cells spontaneously released IL-6 in culture. IL-1 beta and tumour necrosis factor (TNF)-alpha (0.1-100.0 ng/ ml) potentiated IL-y secretion in a time- and dose-dependent manner. Interferon-gamma (0.4-400 ng/ml) induced a dose-related increase in IL-6 secretion and showed a synergistic effect on that secretion in combination with TNF-alpha (10 ng/ ml). Either spontaneous or cytokine-induced IL-6 secretion was inhibited by progesterone (10(-8)-10(-5) M) and danazol (10(-6) M), whereas oestradiol (10(-8)-10(-5) M) had a limited inhibitory effect. The antiprogestin RU486 (10(-8)-10(-4) M) antagonized the inhibitory effects of progesterone and danazol, but showed agonist action when used alone. These findings indicate that endometriotic tissue may actively contribute to the biological changes observed in the peritoneal fluid of endometriosis patients. They also provide new insights into the mechanisms of action of progesterone and those of danazol and RU486 used in the treatment of endometriosis.
My notes (saved in your browser only)
Condition tags
MeSH descriptors
Citation neighborhood
Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.
Cited by (50)
- Letrozole plus metformin versus letrozole alone in the treatment of symptomatic endometrioma 2025
- The association between genetically predicted systemic inflammatory regulators and endometriosis: A bidirectional Mendelian randomization study 2024
- Pathogenesis of Endometriosis: Role of Platelets in Endometriosis 2022
- Immunopathogenesis of endometriosis: An overview of the role of innate and adaptive immune cells and their mediators 2022
- Epigenetic regulation and T-cell responses in endometriosis – something other than autoimmunity 2022
- Effects of metformin, letrozole and atorvastatin on inflammation and apoptosis in experimental peritoneal and ovarian endometriosis in the rat 2022
- Pyrvinium pamoate induces in-vitro suppression of IL-6 and IL-8 produced by human endometriotic stromal cells 2020
- Endometriotic lesions 2019
- Sphingosine I Phosphate (S1P) Increased IL-6 Expression and Cell Growth in Endometriotic Cells 2019
- Daidzein-rich isoflavone aglycones inhibit cell growth and inflammation in endometriosis 2018
- Dysfunctional signaling underlying endometriosis: current state of knowledge 2018
- Serum miR-17, IL-4, and IL-6 levels for diagnosis of endometriosis 2018
- Hypoxia‐inhibited DUSP2 expression promotes IL‐6/STAT3 signaling in endometriosis 2017
- Early Endometriosis in Females Is Directed by Immune-Mediated Estrogen Receptor α and IL-6 Cross-Talk 2017
- Circulating and peritoneal fluid interleukin-6 levels and gene expression in pelvic endometriosis 2017
- PRL-3 Is Involved in Estrogen- and IL-6–Induced Migration of Endometrial Stromal Cells From Ectopic Endometrium 2016
- Endometriosis-Derived Stromal Cells Secrete Thrombin and Thromboxane A2, Inducing Platelet Activation 2016
- Tranylcypromine, a lysine-specific demethylase 1 (LSD1) inhibitor, suppresses lesion growth and improves generalized hyperalgesia in mouse with induced endometriosis 2016
- Augmented Angiogenic Factors Expression via FP Signaling Pathways in Peritoneal Endometriosis 2016
- Pain related genes in endometriosis: A meta-analysis 2015
- Dual suppression of estrogenic and inflammatory activities for targeting of endometriosis 2015
- P-selectin as a potential therapeutic target for endometriosis 2015
- A comparison between serum levels of interleukin-6 and CA125 in patients with endometriosis and normal women. 2015
- Non‐hormonal targets underlying endometriosis: A focus on molecular mechanisms 2015
- Peptide inhibitors of angiogenesis in endometriosis and the female reproductive system 2014
- Macrophages in Pathophysiology of Endometriosis 2014
- Inflammation and Cytokines in Endometriosis 2014
- Endometriosis and Immunology 2013
- Identification of multiple and distinct defects in prostaglandin biosynthetic pathways in eutopic and ectopic endometrium of women with endometriosis 2013
- Enhanced Inflammatory Activity of Endometriotic Lesions from the Rectovaginal Septum 2013
- Metformin as a new therapy for endometriosis, its effects on both clinical picture and cytokines profile 2012
- Endometriosis and Angiogenic Factors 2012
- Effect of antiangiogenic treatment on peritoneal endometriosis-associated nerve fibers 2012
- Neuroimmunomodulatory Alterations in Non-Lesional Peritoneum Close to Peritoneal Endometriosis 2012
- Pelvic Mechanisms Involved in the Pathophysiology of Pain in Endometriosis 2011
- Distinct expression of the soluble and the membrane-bound forms of interleukin-1 receptor accessory protein in the endometrium of women with endometriosis 2011
- Activin-A is induced by interleukin-1β and tumor necrosis factor-α and enhances the mRNA expression of interleukin-6 and protease-activated receptor-2 and proliferation of stromal cells from endometrioma 2011
- ORIGINAL ARTICLE: TNFα Gene Silencing Reduced Lipopolysaccharide‐Promoted Proliferation of Endometriotic Stromal Cells 2009
- Endometriosis, assisted reproduction technology, and risk of adverse pregnancy outcome 2009
- IVF outcome in women with endometriosis in relation to tumour necrosis factor and anti-Müllerian hormone 2009
- Endometriosis and ovarian reserve : Inflammation and prognostic markers 2008
- Role of melatonin in regulating matrix metalloproteinase‐9 via tissue inhibitors of metalloproteinase‐1 during protection against endometriosis 2008
- Cytokine profiles in serum and peritoneal fluid from infertile women with and without endometriosis 2007
- Trichostatin A, a Histone Deacetylase Inhibitor, Attenuates Invasiveness and Reactivates E-Cadherin Expression in Immortalized Endometriotic Cells 2007
- Metformin Suppresses Interleukin (IL)-1β-Induced IL-8 Production, Aromatase Activation, and Proliferation of Endometriotic Stromal Cells 2007
- Stable inhibition of interleukin 1 receptor type II in Ishikawa cells augments secretion of matrix metalloproteinases: possible role in endometriosis pathophysiology 2007
- Imbalance in the expression of the activating type I and the inhibitory type II interleukin 1 receptors in endometriosis 2007
- Paracrine regulation of endometriotic tissue 2007
- Increased expression of interleukin-1 receptor type 1 in active endometriotic lesions 2007
- Human chorionic gonadotrophin attenuates NF-κB activation and cytokine expression of endometriotic stromal cells 2007
Source provenance
- europepmc
- last seen: 2026-06-04T01:30:01.192114+00:00
- openalex
- last seen: 2026-06-10T17:14:06.276822+00:00
- pubmed
- last seen: 2026-05-13T22:10:57.821266+00:00
License: CC0
· commercial use OK