Peritoneal endometriosis: validation of an in-vivo model

R Grümmer, Ruth Grümmer, F Schwarzer, K Bainczyk, H Hess-Stumpp, P A Regidor, A E Schindler, E Winterhager
Human Reproduction · 2001 · vol. 16(8) , pp. 1736–1743 · doi:10.1093/humrep/16.8.1736 · PMID:11473975 · W2160909556
article OA: bronze CC0 ⤵ 71 in-corpus citations
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This study validated a nude mouse model for intraperitoneal cultivation of human endometrial tissue, demonstrating lesion persistence, epithelial proliferation, and neoangiogenesis for up to 28 days.

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Abstract

BACKGROUND: The current medical treatment of endometriosis, a common gynaecological disease, is still associated with a high recurrence rate. To establish an appropriate in-vivo model to evaluate new therapeutic strategies we validated the nude mouse model for the intraperitoneal cultivation of human endometrial tissue. METHODS: Human endometrium of the proliferative phase was implanted into the peritoneal cavity of normal cycling and ovariectomized athymic mice and of cycling non-obese diabetic (NOD)-severe combined immuno-deficiency (SCID) mice. Morphology, proliferation, differentiation, and angiogenesis in the ectopic endometrium at different time points after implantation was investigated. RESULTS: Adhesion of endometrial fragments was observed from day 2 onwards. The lesions persisted for up to 28 days revealing a well preserved glandular morphology. The glandular epithelium maintained cytokeratin expression even after 14 days of culture. With progressing culture, glands exhibited vimentin staining in combination with a decrease of surrounding stromal cells. Proliferation of glandular epithelium could be demonstrated throughout the investigated period of 28 days, whereas expression of oestrogen and progesterone receptors was maintained only in endometriotic lesions grown in cycling but not in ovariectomized mice. Neoangiogenesis occurred from day 4 onwards, independent from the intraperitoneal localization of the ectopic lesions. CONCLUSIONS: This in-vivo model is a promising tool to test the effect of compounds such as different hormone agonists/antagonists or anti-angiogenic factors to develop new therapeutic concepts in endometriosis.

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Condition tags

endometriosis

MeSH descriptors

Disease Models, Animal Endometriosis Endometriosis Endometriosis Endometrium Peritoneal Diseases Peritoneal Diseases Peritoneal Diseases Adult Animals Cell Differentiation Cell Division Endometrium Female Humans Immunohistochemistry Keratins Keratins Ki-67 Antigen Ki-67 Antigen

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