Luminescence as a Tool to Assess Pelvic Endometriosis Development in Murine Models

Classic murine endometriosis models may be insufficient to evaluate the effect of therapeutic agents on endometriosis development, because the process of identification and measurement of induced lesions is often impeded, as implants are small and embedded in murine tissue. In this context, as summarized in the current review, luminescence techniques have proved useful for identifying and visualizing or quantifying endometriotic transplants. They are also a valuable tool for endometrial cell tracking in live animals, yielding further information by adding spatial and temporal dimensions to biological processes in vivo. Such approaches involve transplanting luminescently labeled murine or human endometrium into animals. Two main strategies are applied to label endometrium before injection: use of genetically modified tissue or tissue labeled with a fluorescent dye. Each model has its advantages and disadvantages, the choice of model depends on the study objectives/design (long- or short-term studies, homologous or heterologous model). Similar content being viewed by others

Sampson J. Peritoneal endometriosis is due to the menstrual dissemination of endometrial tissue into the peritoneal cavity. Am J Obstet Gynecol. 1927;14:433–469. D’Hooghe TM. Clinical relevance of the baboon as a model for the study of endometriosis. Fertil Steril. 1997;68(4):613–25. Schenken RS, Asch RH. Surgical induction of endometriosis in the rabbit: effects on fertility and concentrations of peritoneal fluid prostaglandins. Fertil Steril. 1980;34(6):581–587. Cummings AM, Metcalf JL. Induction of endometriosis in mice: a new model sensitive to estrogen. Reprod Toxicol. 1995;9(3):233–238. Foster WG, Ruka MP, Gareau P, Foster RA, Janzen EG, Yang JZ. Morphologic characteristics of endometriosis in the mouse model: application to toxicology. Can J Physiol Pharmacol. 1997;75(10–11):1188–1196. Vernon MW, Wilson EA. Studies on the surgical induction of endometriosis in the rat. Fertil Steril. 1985;44(5):684–694. Sharpe-Timms KL. Using rats as a research model for the study of endometriosis. Ann N Y Acad Sci. 2002;955:318–327. Aoki D, Katsuki Y, Shimizu A, Kakinuma C, Nozawa S. Successful heterotransplantation of human endometrium in scid mice. Obstet Gynecol. 1994;83(2):220–228. Awwad JT, Sayegh RA, Tao XJ, Hassan T, Awwad ST, Isaacson K. The scid mouse: an experimental model for endometriosis. Hum Reprod. 1999;14(12):3107–3111. Grummer R, Schwarzer F, Bainczyk K, et al. Peritoneal endometriosis: validation of an in-vivo model. Hum Reprod. 2001;16(8):1736–1743. Zamah NM, Dodson MG, Stephens LC, Buttram VC Jr, Besch PK, Kaufman RH. Transplantation of normal and ectopic human endometrial tissue into athymic nude mice. Am J Obstet Gynecol. 1984;149(6):591–597. Nisolle M, Casanas-Roux F, Donnez J. Early-stage endometriosis: adhesion and growth of human menstrual endometrium in nude mice. Fertil Steril. 2000;74(2):306–312. Greenberg LH, Slayden OD. Human endometriotic xenografts in immunodeficient rag-2/gamma(c)ko mice. Am J Obstet Gynecol. 2004;190(6):1788–1795. Bambot SB, Lakowicz JR, Rao G. Potential applications of lifetime-based, phase-modulation fluorimetry in bioprocess and clinical monitoring. Trends Biotechnol. 1995;13(3):106–115. Kaijzel EL, van der Pluijm G, Lowik CW. Whole-body optical imaging in animal models to assess cancer development and progression. Clin Cancer Res. 2007;13(12):3490–3497. Mehta SR, Huang R, Yang M, et al. Real time in vivo gfp imaging in a murine leishmaniasis model: a new tool for leishmania vaccine and drug discovery. Clin Vaccine Immunol. 2008;15(12):1764–1770. Neff BA, Voss SG, Allen C, et al. Bioluminescent imaging of intracranial vestibular schwannoma xenografts in nod/scid mice. Otol Neurotol 2008;30(1):105–111. Honig MG, Hume RI. Dil and dio: Versatile fluorescent dyes for neuronal labelling and pathway tracing. Trends Neurosci. 1989;12(9):333–335, 340–341. Cong L, Sun D, Zhang Z, Jiao W, Rizzolo LJ, Peng S. A novel rabbit model for studying rpe transplantation. Invest Ophthalmol Vis Sci. 2008;49(9):4115–4125. Malik E, Berg C, Meyhöfer-Malik A, Buchweitz O, Moubayed P, Diedrich K. Fluorescence diagnosis of endometriosis using 5-aminolevulinic acid. Surg Endosc. 2000;14(5):452–455. Hillemanns P, Weingandt H, Stepp H, Baumgartner R, Xiang W, Korell M. Assessment of 5-aminolevulinic acid-induced porphyrin fluorescence in patients with peritoneal endometriosis. Am J Obstet Gynecol. 2000;183(1):52–57. Buchweitz O, Wulfing P, Staebler A, Kiesel L. Detection of nonpigmented endometriotic lesions with 5-aminolevulinic acid-induced fluorescence. J Am Assoc Gynecol Laparosc. 2004;11(4):505–510. Demco L. Laparoscopic spectral analysis of endometriosis. J Am Assoc Gynecol Laparosc. 2004;11(2):219–222. Buchweitz O, Staebler A, Tio J, Kiesel L. Detection of peritoneal endometriotic lesions by autofluorescence laparoscopy. Am J Obstet Gynecol. 2006;195(4):949–954. Tabibzadeh S, Miller S, Dodson WC, Satyaswaroop PG. An experimental model for the endometriosis in athymic mice. Front Biosci. 1999;4:4–9. Nap AW, Groothuis PG, Demir AY, Evers JL, Dunselman GA. Pathogenesis of endometriosis. Best Pract Res Clin Obstet Gynaecol. 2004;18(2):233–244. Illera MJ, Juan L, Stewart CL, Cullinan E, Ruman J, Lessey BA. Effect of peritoneal fluid from women with endometriosis on implantation in the mouse model. Fertil Steril. 2000;74(1):41–48. Hull ML, Charnock-Jones DS, Chan CL, et al. Antiangiogenic agents are effective inhibitors of endometriosis. J Clin Endocrinol Metab. 2003;88(6):2889–2899. Van Langendonckt A, Casanas-Roux F, Eggermont J, Donnez J. Characterization of iron deposition in endometriotic lesions induced in the nude mouse model. Hum Reprod. 2004;19(6):1265–1271. Somigliana E, Vigano P, Rossi G, Carinelli S, Vignali M, Panina-Bordignon P. Endometrial ability to implant in ectopic sites can be prevented by interleukin-12 in a murine model of endometriosis. Hum Reprod. 1999;14(12):2944–2950. Fortin M, Lepine M, Page M, et al. An improved mouse model for endometriosis allows noninvasive assessment of lesion implantation and development. Fertil Steril. 2003;80(suppl 2):832–838. Hirata T, Osuga Y, Yoshino O, et al. Development of an experimental model of endometriosis using mice that ubiquitously express green fluorescent protein. Hum Reprod. 2005;20(8):2092–2096. Defrere S, Van Langendonckt A, Gonzalez Ramos R, Jouret M, Mettlen M, Donnez J. Quantification of endometriotic lesions in a murine model by fluorimetric and morphometric analyses. Hum Reprod. 2006;21(3):810–817. Becker CM, Wright RD, Satchi-Fainaro R, et al. A novel noninvasive model of endometriosis for monitoring the efficacy of antiangiogenic therapy. Am J Pathol. 2006;168(6):2074–2084. Masuda H, Maruyama T, Hiratsu E, et al. Noninvasive and real-time assessment of reconstructed functional human endometrium in nod/scid/gamma c(null) immunodeficient mice. Proc Natl Acad Sci USA. 2007;104(6):1925–1930. Becker CM, Rohwer N, Funakoshi T, et al. 2-methoxyestradiol inhibits hypoxia-inducible factor-1{alpha} and suppresses growth of lesions in a mouse model of endometriosis. Am J Pathol. 2008;172(2):534–544. Fortin M, Lepine M, Merlen Y, et al. Quantitative assessment of human endometriotic tissue maintenance and regression in a noninvasive mouse model of endometriosis. Mol Ther. 2004;9(4):540–547. Defrere S, Van Langendonckt A, Vaesen S, et al. Iron overload enhances epithelial cell proliferation in endometriotic lesions induced in a murine model. Hum Reprod. 2006;21(11):2810–2816. Gonzalez-Ramos R, Van Langendonckt A, Defrere S, et al. Agents blocking the nuclear factor-kappab pathway are effective inhibitors of endometriosis in an in vivo experimental model. Gynecol Obstet Invest. 2008;65(3):174–186. Thompson JF, Hayes LS, Lloyd DB. Modulation of firefly luciferase stability and impact on studies of gene regulation. Gene. 1991;103(2):171–177. Spinelli SV, Rodriguez JV, Quintana AB, Mediavilla MG, Guibert EE. Engraftment and function of intrasplenically transplanted cold stored rat hepatocytes. Cell Transplant. 2002;11(2):161–168. Author information Authors and Affiliations Corresponding author Rights and permissions About this article Cite this article Defrère, S., Colette, S., Lousse, JC. et al. Luminescence as a Tool to Assess Pelvic Endometriosis Development in Murine Models. Reprod. Sci. 16, 1117–1124 (2009). https://doi.org/10.1177/1933719109335069 Published: Issue date: DOI: https://doi.org/10.1177/1933719109335069