A red fluorescent nude mouse model of human endometriosis: advantages of a non-invasive imaging method

Wang N, Ningning Wang, Shanshan Hong, Hong S, Tan J, Jinfeng Tan, Peiqi Ke, Ke P, Lili Liang, Liang L, Hui Fei, Fei H, Liu B, Bin Liu, Liqun Liu, Liu L, Liu Y, Yongdong Liu, Yu B, Bingjun Yu
European journal of obstetrics, gynecology, and reproductive biology · 2014 · vol. 176 , pp. 25–30 · doi:10.1016/j.ejogrb.2014.02.012 · PMID:24630298 · W2093721139
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Researchers developed a red fluorescent nude mouse model to non-invasively image human endometriosis, demonstrating advantages over traditional assessment methods.

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Abstract

ObjectivesTo establish red fluorescent human endometriosis lesions in a nude mouse model and dynamically and non-invasively to compare intraperitoneal and subcutaneous injection models.Study designPrimary cultures of endometrial stromal cells (ESCs) and epithelial cells (EECs) isolated from 24 patients with a normal uterine cavity were transfected with 2.5×10(8) (Group 1) and 1.25×10(8) (Group 2) plaque-forming units (PFU) of adenovirus encoding red fluorescent protein (Ad-RFP). Transfection efficiencies, fluorescence intensity and apoptosis rate of the two types of cells were compared in vitro. A mixture of 2.5×10(8) PFU Ad-RFP-infected approximately 400 EECs cell mass and 2×10(6) ESCs for 36h was injected individually into 24 female nude mice subcutaneously (Group A) or intraperitoneally (Group B). From Day 5 after injection, an in vivo imaging system (IVIS) was used to non-invasively observe and compare the lesions of the two groups every week until Day 33. Specifically, the fluorescent intensity, positive rates, persistence time and lesion weight in the implanted human endometriosis lesions were compared. A parametric Student's t-test and two-way analysis of variance were used for statistical analysis.ResultsCompared with 1.25×10(8) PFU RFP, a titre of 2.5×10(8) PFU RFP ESCs and EECs incubated for 36h exhibited higher transfection efficiencies and higher fluorescence intensities in vitro. In vivo imaging of the fluorescent human endometriosis lesions originating from an RFP titre of 2.5×10(8) PFU showed that the intensity and lesion weight in Group A were significantly higher than in Group B. However, the two groups had the same RFP-positive rates and fluorescence persistence. The structure of each lesion was evaluated by immunohistochemistry to confirm its human endometrial origin.ConclusionsThe red fluorescent human endometriosis model established by subcutaneously injecting 2.5×10(8) PFU RFP-transfected stromal cells and epithelial cells into nude mice had a higher fluorescent positive rate from Day 5, higher intensity and weight but the same persistence as the intraperitoneal injection model.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Luminescent Proteins Adult Animals Endometriosis Epithelial Cells Epithelial Cells Female Humans Injections, Subcutaneous Luminescent Proteins Mice, Nude Middle Aged Red Fluorescent Protein Stromal Cells Stromal Cells Transfection

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organisms 13
transgenic mice human human transgenic mice unidentified adenovirus amylotrama banrockensis amylotrama banrockensis transgenic mice human human human human transgenic mice

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