Establishment of a model of endometriosis in vitro with endometrial cells expressing green fluorescent protein

Objective To reproduce a model of endometriosis with endometrial cells expressing green fluorescent protein(GFP) in vitro induced by adenovirus.Methods Five batches of normal endometrial samples were isolated and cultured,morphologically observed and identied with immunofluorescence method.The isolated endometrial cells were then transfected with green fluorescent protein(GFP) gene-associated adenovirus.GFP positive rate and apoptosis rate were determined by flow cytometry,and they were compared 12,18,36,and 42 hours after transfection.Results The endometrial glandular cells and stromal cells were successfully isolated in high purity(90% in glandular cells and approximately 100% in stromal cells).Green fluorescence protein was expressed in the both types of infected cells.Compared with endometrial glandular cells,GFP positive rate in stromal cells was higher(F=8.362,P=0.020) and apoptosis rate was lower(F=46.119,P0.001);transfection factor threw no significant influence on the apoptosis of the cells(F=4.208,P=0.057).Conclusion A stable in vitro fluorescent model of endometriosis can be reproduced by transfecting endometrial glandular and stromal cells with adenovirus-encoded GFP gene.