Elevated expression of tumour necrosis factor α in cultured granulosa cells from women with endometriosis

Magdalena Carlberg, M Carlberg, Jelve Nejaty, J Nejaty, Berit Fröysa, B Fröysa, Yongmei Guan, Y Guan, Olle Söder, O Söder, A Bergqvist, Agneta Bergqvist
Human Reproduction · 2000 · vol. 15(6) , pp. 1250–1255 · doi:10.1093/humrep/15.6.1250 · PMID:10831550 · W1981389194
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Granulosa cells from women with endometriosis showed significantly elevated basal production of tumor necrosis factor alpha compared to controls undergoing IVF.

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Abstract

Fertilization and oocyte cleavage rates have previously been demonstrated to be lower for women with endometriosis undergoing IVF compared with controls. This might be related to impaired oocyte function, possibly due to an inflammatory milieu in the pelvis of these women, where an elevated concentration of many cytokines is documented. The aim of this study was to examine whether granulosa cells from women with endometriosis deviated with respect to production of the inflammatory cytokines interleukin-1beta, interleukin-6, interleukin-8 and tumour necrosis factor alpha (TNFalpha) compared with granulosa cells from healthy women, undergoing IVF for male infertility. The effect of human chorionic gonadotrophin on cytokine production was also investigated. Granulosa cells in follicular fluid were obtained at oocyte retrieval for IVF. Incubated cell culture media were analysed by enzyme-linked immunosorbent assay. The basal production of all four cytokines was higher in cells from women with endometriosis when compared to controls, although the increase was only significant for TNFalpha. Chorionic gonadotrophin had no significant effect, although it had a tendency to suppress cytokine release in both patient categories. Whether aberrant cytokine production in granulosa cells from women with endometriosis may disturb fertilizing capacity of oocytes requires study.

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Condition tags

mesh:D004715endometriosisinfertility

MeSH descriptors

Endometriosis Endometriosis Granulosa Cells Tumor Necrosis Factor-alpha Adult Cells, Cultured Chorionic Gonadotropin Chorionic Gonadotropin Cytokines Cytokines Endometriosis Female Fertilization in Vitro Granulosa Cells Humans Infertility, Female Infertility, Female Infertility, Male Infertility, Male Interleukin-1

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