Interleukin-8 concentration in peritoneal fluid of patients with endometriosis and modulation of interleukin-8 expression in human mesothelial cells

Aydin Arici, Arici A, A Arici, Tazuke SI, Salli I. Tazuke, S I Tazuke, E Attar, Harvey J. Kliman, Attar E, David L. Olive, H J Kliman, Kliman HJ, D L Olive, Olive DL
Molecular human reproduction · 1996 · vol. 2(1) , pp. 40–45 · doi:10.1093/molehr/2.1.40 · PMID:9238656 · W2051981970
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Interleukin-8 concentrations in peritoneal fluid are elevated in women with endometriosis, and cultured mesothelial cells produce IL-8 in response to inflammatory cytokines.

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Abstract

Interleukin-8 (IL-8) is a chemoattractant and activating factor for human neutrophlls and a potent angiogenic agent. The peritoneal fluid of women with endometriosis has been shown to have increased neutrophil chemotactic activity. We postulate that IL-8 may be an important modulator in the pathogenesis of endometriosis and adhesion formation. We first investigated IL-8 concentrations in the peritoneal fluid of women with or without endometriosis, then assessed peritoneal mesothelial cells as a potential source of peritoneal fluid IL-8. Northern blot analysis and enzyme-linked immunosorbent assay (ELISA) were used to investigate IL-8 mRNA and protein modulation. The mean concentration of IL-8 in samples obtained from control patients (n = 28) was 4.8 +/- 0.5 pg/ml; from patients with minimal-mild endometriosis (n = 24) was 27.5 +/- 2.6 pg/ml; and from patients with moderate-severe endometriosis (n = 21) was 530.2 +/- 65.1 pg/ml. Confluent mesothelial cells were incubated with human recombinant IL-1 alpha (0.01-100 IU/ml) or tumour necrosis factor (TNF)-alpha (0.01 to 100 ng/ml) for 2-24 h. IL-8 mRNA was detectable in non-treated cells, however both IL-1 alpha and TNF-alpha induced higher amounts of IL-8 mRNA in a dose- and time-dependent manner. Non-treated mesothelial cells in culture also produced and secreted IL-8 protein quantified by ELISA, but again higher concentrations were induced by IL-1 alpha and TNF-alpha treatment. In conclusion, we found that IL-8 concentrations were elevated in peritoneal fluids from women with endometriosis. Cultured mesothelial cells expressed cytokine-inducible IL-8 mRNA and secreted IL-8 protein. The regulated expression of this angiogenic factor may play a role in pathogenesis of endometriosis.

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Condition tags

endometriosis

MeSH descriptors

Ascitic Fluid Endometriosis Interleukin-8 Ascitic Fluid Ascitic Fluid Ascitic Fluid Ascitic Fluid Blotting, Northern Blotting, Northern Cells, Cultured Cells, Cultured Endometriosis Endometriosis Endometriosis Endometriosis Epithelium Epithelium Epithelium Epithelium Female

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