Role of Versican in the Pathogenesis of Peritoneal Endometriosis

Hirohiko Tani, Yukiyasu Sato, Masashi Ueda, Yumiko Miyazaki, Koh Suginami, Akihito Horie, Ikuo Konishi, Tamayuki Shinomura
The Journal of clinical endocrinology and metabolism · 2016 · vol. 101(11) , pp. 4349–4356 · doi:10.1210/jc.2016-2391 · PMID:27533311 · W2511544623
article OA: bronze CC0 ⤵ 36 in-corpus citations
📄 Open PDF View on OpenAlex View on PubMed View at publisher

Abstract

CONTEXT: Sampson's theory cannot explain why only some cycling women develop peritoneal endometriosis. Few studies have focused on the pelvic peritoneum, which receives regurgitated endometrial tissues. We hypothesized that molecular alterations in the peritoneum are involved in the development of peritoneal endometriosis and conducted a microarray analysis to compare macroscopically normal peritoneum sampled from women with peritoneal endometriosis (endometriotic peritoneum) and those without (non-endometriotic peritoneum). Versican, a major proteoglycan component of the extracellular matrix, is one of the molecules up-regulated in endometriotic peritoneum. OBJECTIVE: To investigate the role of versican in peritoneal endometriosis. Design, Patients, and Main Outcome Measure: Endometriotic peritoneum and non-endometriotic peritoneum were subjected to RT-PCR, immunostaining, and Western blotting. The versican V1 isoform was stably transfected into Chinese hamster ovary cells (CHO-V1), and the effects of CHO-V1-derived conditioned medium (V1-CM) on primary human endometrial stromal cells were investigated with attachment, invasion, and proliferation assays. The effects of peritoneal fluid collected from endometriotic women (endometriotic PF) or cytokines/growth factors, which were shown to be elevated in endometriotic PF, on versican expression in a human peritoneal cell line (HMrSV5) were also examined. RESULTS: Versican V1 expression levels were significantly higher in endometriotic peritoneum. In vitro, V1-CM promoted attachment to the HMrSV5 cell monolayer as well as the Matrigel invasion of endometrial stromal cells. Although versican V1 expression was up-regulated by TGF-β1 in HMrSV5 cells, it remained unchanged in endometriotic PF. CONCLUSIONS: Our results suggest the involvement of peritoneal versican in the development of peritoneal endometriosis.

My notes (saved in your browser only)

Condition tags

endometriosis

MeSH descriptors

Ascitic Fluid Endometriosis Peritoneal Diseases Versicans Adult Animals Ascitic Fluid Cell Line CHO Cells Cricetulus Endometriosis Female Humans Middle Aged Peritoneal Diseases Protein Isoforms Up-Regulation Versicans

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (30)

Cited by (36)

Source provenance

europepmc
last seen: 2026-06-19T06:14:56.452680+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
pubmed
last seen: 2026-05-13T22:20:43.714878+00:00
License: CC0 · commercial use OK