Immunohistochemical Evaluation of Potential Biomarkers for Targeted Intraoperative Fluorescence Imaging in Endometriosis: Towards Optimizing Surgical Treatment
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Immunohistochemistry revealed MMP11 and VCAN are upregulated in endometriosis tissues, showing membranous or extracellular staining patterns, making them promising targets for fluorescence-guided surgical visualization.
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Abstract
Surgical intervention for endometriosis is an important treatment modality, yet incomplete resection resulting from poor visibility of affected tissue and consequently recurrence of disease remains a prevalent challenge. Intra-operative visualization of endometriosis, enabling fluorescence-guided surgery (FGS), could help to optimize surgical treatment. A biomarker, upregulated in endometriosis compared to adjacent tissue, is required to use as a target for FGS. Immunohistochemistry was used to evaluate protein expression of a selection of previously identified potential biomarkers. Ten biomarkers were stained in a large cohort of 84 tissues, both deep and peritoneal endometriosis and tissue without endometriosis, all from patients with confirmed endometriosis. MMP11 and VCAN showed the largest upregulation in endometriosis compared to adjacent tissue and showed a membranous or extracellular staining pattern. MMP11 is a promising target for glandular and stromal visualization, VCAN for stromal visualization only. For both biomarkers, upregulation was high in both peritoneal and deep endometriosis and for patients with and without hormonal medication. Other stained biomarkers showed non-beneficial characteristics based on staining pattern or upregulation. Analysis of all endometriosis samples showed that combined glandular and stromal targeting is expected to result in optimal visualization of endometriosis. Further research is needed to determine whether targeting one biomarker is sufficient for this goal, or if dual targeting is necessary. Development of clinical tracers for VCAN and MMP11 is necessary.
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References (33)
- Analysis of biomarker expression in severe endometriosis and determination of possibilities for targeted intraoperative imaging via openalex
- Analysis of differences in the transcriptomic profiles of eutopic and ectopic endometriums in women with ovarian endometriosis via openalex
- Assessing research gaps and unmet needs in endometriosis via openalex
- Biomarker identification for endometriosis as a target for real-time intraoperative fluorescent imaging: A new approach using transcriptomic analysis to broaden the search for potential biomarkers via openalex
- Classification of deep endometriosis (DE) including bowel endometriosis: From r-ASRM to #Enzian-classification via openalex
- Endometriosis: Cellular and Molecular Mechanisms Leading to Fibrosis via openalex
- Epidemiology of endometriosis and its comorbidities via openalex
- Eutopic endometrium and peritoneal, ovarian and bowel endometriotic tissues express a different profile of matrix metalloproteinases-2, -3 and -11, and of tissue inhibitor metalloproteinases-1 and -2 via openalex
- Expression of Matrix Metalloproteinases and Their Inhibitors in Endometrium: High Levels in Endometriotic Lesions via openalex
- Model Systems in Endometriosis Research: Translation, Translation, Translation! via openalex
- Optimal Management of Endometriosis and Pain via openalex
- Recurrence after Surgery for Colorectal Endometriosis: A Systematic Review and Meta-analysis via openalex
- Recurrence in Deep Infiltrating Endometriosis: A Systematic Review of the Literature via openalex
- Role of Versican in the Pathogenesis of Peritoneal Endometriosis via openalex
- The Role of Matrix Metalloproteinases in Endometriosis: A Potential Target via openalex
- Time to redefine endometriosis including its pro-fibrotic nature via openalex
- W1840865169 via openalex
- W4386160360 via openalex
- W2026762108 via openalex
- W2056710301 via openalex
- W2077787084 via openalex
- W2113630000 via openalex
- W2115223685 via openalex
- W2129591711 via openalex
- W2755010202 via openalex
- W2765399611 via openalex
- W2955213391 via openalex
- W2967419092 via openalex
- W3019034047 via openalex
- W3196963730 via openalex
- W3217410954 via openalex
- W4206519465 via openalex
- W4367676419 via openalex
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