Peritoneal fluid from women with endometriosis impairs human spermatozoa functionality

Paula Sáez‐Espinosa, Paula Sáez-Espinosa, Velasco I, Irene Velasco, Lorca P, Patricia Lorca, María Isabel Acién, Maribel Acién, Alejandro Romero, María José Gómez‐Torres, María José Gómez-Torres
Reproductive biology · 2019 · vol. 20(1) , pp. 81–87 · doi:10.1016/j.repbio.2019.12.001 · PMID:31864823 · W2995532217
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Peritoneal fluid from women with endometriosis was found to reduce human sperm motility and viability.

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Abstract

The success of mammalian fertilization depends largely on spermatozoa physiological events. However, the manner in which endometriosis influence morpho-functional spermatozoa biomarkers is poorly defined. Here, we studied in vitro the effect of peritoneal fluid (PF) from women with endometriosis (PFE) and non-endometriosis (PFNE) on spermatozoa parameters. This research was prospective and double-blind. A total of 45 PF samples were collected from women with (n = 25) and without endometriosis (n = 20). Semen samples were obtained from normozoospermic donors (n = 15) and cultured with 20 % (v/v) of PF or commercial culture medium (controls) during 0, 24, and 48 h. The outcome measures were spermatozoa/viability, motility, tyrosine phosphorylation (TP) and spontaneous acrosomal reaction. In addition, plasma membrane sugars were characterized by lectins [Aleuria aurantia agglutinin (AAA), Concanavalin A (ConA), Peanut agglutinin (PNA), and Wheat germ agglutinin (WGA)]. After a 24 -h culture, results reported a significant decrease in motility in cells cultured with PFE compared to the control, together with differences in the AAA and WGA-binding sites. Moreover, spermatozoa in contact with PFNE presented a significantly lower level of acrosome spontaneous reaction. At 48 h, no differences were observed in the biomarkers studied between the PFNE and the control, excluding ConA-binding sites. On the other hand, cells cultured with PFE exhibited significantly less motility, TP, and differences in the relocation of spermatozoa surface sugars. Viability was not affected in any culture condition. Overall, the effects of PFE could negatively affect spermatozoa quality, contributing to explain and diagnose the infertility associated to male partners of women with endometriosis.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Infertility Spermatozoa Acrosome Reaction Adult Ascitic Fluid Case-Control Studies Culture Media, Conditioned Endometriosis Female Humans Infertility Male Middle Aged Sperm Motility

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (48)

Cited by (3)

SciLite annotations

organisms 4
human peziza aurantia peanut wheat
chemicals 4
tyrosine fluoro sugar pyropheophorbide a fluoro sugar

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