Dynamics of nitric oxide, altered follicular microenvironment, and oocyte quality in women with endometriosis

Pravin T Goud, Goud PT, A.P. Goud, Goud AP, Anuradha P Goud, Narendra Joshi, Joshi N, Puscheck E, Elizabeth E. Puscheck, Elizabeth Puscheck, Michael P Diamond, Husam M. Abu‐Soud, Husam M Abu-Soud
Fertility and sterility · 2014 · vol. 102(1) , pp. 151–159.e5 · doi:10.1016/j.fertnstert.2014.03.053 · PMID:24825428 · W2040726024
article OA: closed CC0 ⤵ 61 in-corpus citations
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This study investigated the impact of nitric oxide levels and the resulting follicular microenvironment changes on oocyte quality in women diagnosed with endometriosis.

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Abstract

ObjectiveTo study follicular microenvironment in terms of free radical dynamics, oocyte quality, and assisted reproductive technology (ART) outcomes among women with (group A) and without (group B) endometriosis.DesignProspective cohort study.SettingUniversity ART center.Patient(s)Women with and without endometriosis undergoing ART (n=28).Intervention(s)Follicular fluid (FF), granulosa cells (GCs), immature oocytes (IOs), and ART data on sibling cohort oocytes in groups A and B were compared.Main outcome measure(s)ART live birth outcomes, maturation, and aging among in vitro matured (IVM) oocytes, nitrate levels in FF, and nitrotyrosine (NT) footprints and apoptosis in the GCs.Result(s)Clinical characteristics and ART live birth outcomes were no different between groups A and B. Women from group A had significantly lower peak serum E2 (2,068.8±244.6 pg/mL vs. 2,756.2±205.0 pg/mL) and higher apoptosis (80.0% vs. 22.2%) and NT staining (70.0% vs. 22.2%) in GCs compared with group B. Fewer IOs underwent IVM to MII (0.6±0.3) in group A compared with group B (1.4±0.2). IVM oocytes had significantly higher incidence of cortical granule loss (83.3% vs. 24.0%) and spindle disruption (66.7% vs. 16.0%) and higher zona pellucida dissolution timing (133.8±9.4 s vs. 90.5±5.8 s) in group A compared with group B. FF nitrate levels were significantly higher in women who failed to conceive in group A (478.2±43.1 nmol/L) compared with those that did conceive (173.3±19.0 nmol/L).Conclusion(s)Increased protein nitration, GC apoptosis, resistance to IVM, and oocyte aging indicate the involvement of oxidative dysregulation of NO in the pathophysiology of altered follicular milieu and poor oocyte quality in women with endometriosis.

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Condition tags

endometriosis

MeSH descriptors

Cellular Microenvironment Endometriosis Infertility, Female Nitric Oxide Oocytes Ovarian Follicle Adult Apoptosis Biomarkers Biomarkers Case-Control Studies Cellular Senescence Endometriosis Endometriosis Endometriosis Estradiol Estradiol Female Follicular Fluid Follicular Fluid

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Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

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