Exposure to Mono-n-Butyl Phthalate in Women with Endometriosis and Its Association with the Biological Effects on Human Granulosa Cells

Ya-Ching Chou, Yu‐Chun Chen, Yu-Chun Chen, Ming-Jer Chen, Ching-Wen Chang, Ching‐Wen Chang, Guan-Lin Lai, Chii-Ruey Tzeng, Chii‐Ruey Tzeng
International journal of molecular sciences · 2020 · vol. 21(5) , pp. 1794 · doi:10.3390/ijms21051794 · PMID:32151056 · PMC7084286 · W3009913290
article OA: gold CC0 ⤵ 8 in-corpus citations
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Higher urinary mono-n-butyl phthalate levels were found in women with endometriosis, and this metabolite altered gene expression, mitochondrial function, and hormone production in human granulosa cells.

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Abstract

To study the association between urinary phthalate metabolite levels, endometriosis, and their effects on human granulosa cells, we recruited patients who underwent laparoscopy to confirm endometriosis (n = 123) and control patients (n = 78). Liquid chromatography-tandem mass spectrometry was used to measure the following five urinary phthalate metabolites: mono-n-butyl phthalate (MnBP), mono(2-ethylhexyl) phthalate, monobenzyl phthalate, mono(2-ethyl-5-oxo-hexyl) phthalate, and mono(2-ethyl-5-hydroxyhexyl) phthalate. Urinary MnBP levels were higher in patients with endometriosis than in controls after multivariable logistic regression including the number of deliveries, body mass index, and use of medicine as covariables. MnBP correlates with other phthalate metabolites. Previous studies found that endometriosis was a detrimental condition for granulosa cells. In our study, we observed whether MnBP affected granulosa cells. MnBP treatment altered the gene expression of BIRC5, BUB1B, CDC20, cyclin B1, IL-1β, TNF-α, inhibin-B, StAR, and P450ssc and attenuated the ratio of the mitochondrial membrane potential in human granulosa cells. Moreover, MnBP decreased the expression of the anti-Mullerian hormone. These findings suggest that MnBP concentration is associated with endometriosis and may affect the health and steroidogenesis of human granulosa cells.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Environmental Pollutants Granulosa Cells Phthalic Acids Adult Case-Control Studies Endometriosis Endometriosis Environmental Pollutants Female Granulosa Cells Granulosa Cells Humans Phthalic Acids Prognosis

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