Induction of an angiogenic phenotype in endometriotic stromal cell cultures by interleukin-1beta

D I Lebovic, Dan I. Lebovic, Bentzien F, F Bentzien, Frauke Bentzien, Victor A. Chao, V A Chao, Chao Va, Garrett EN, E N Garrett, Evelyn N. Garrett, Meng YG, Y. Gloria Meng, Y G Meng, Robert N. Taylor, Taylor RN, R N Taylor
Molecular human reproduction · 2000 · vol. 6(3) , pp. 269–275 · doi:10.1093/molehr/6.3.269 · PMID:10694276 · W2104653323
Lab / animal OA: bronze CC0 ⤵ 124 in-corpus citations
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Interleukin-1beta induced angiogenic gene and protein expression in endometriotic stromal cells, but not normal endometrial cells, mediated by increased IL-1 receptor expression.

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Abstract

Activated peritoneal macrophages are associated with endometriosis and may play a central role in its aetiology by releasing interleukin-1beta (IL-1beta) in response to refluxed endometrium. Pari passu with the establishment of endometriotic implants is the development of a vascular supply. In this study we investigated the angiogenic properties of two endometrial proteins, vascular endothelial growth factor (VEGF) and interleukin-6 (IL-6), and assessed their production in response to IL-1beta stimulation in human stromal cells isolated from normal endometrium (NE) and endometriotic lesions (EI). Proliferation of bovine brain capillary endothelial cells (BBCE) with a [(3)H]-thymidine incorporation assay was observed when VEGF (2.1 +/- 0.2-fold; P < 0.05) or VEGF and IL-6 (1.8 +/- 0.1-fold; P < 0.05) were added in vitro, relative to saline-treated control cultures. Northern blot analysis showed induction of VEGF mRNA (2.6-fold; P < 0.05) and IL-6 mRNA (6.3-fold; P < 0.05) transcripts in EI cells, but not NE cells, exposed to IL-1beta. A similar induction was seen with VEGF and IL-6 protein secretion in the responsive EI cells. Reverse transcription-polymerase chain reaction (RT-PCR) for the IL-1 receptor type I (IL-1 RI) indicated that the differential effects of IL-1beta on NE and EI cells was associated with 2.4 +/- 0.1-fold more receptor mRNA in EI versus NE cells. We propose that the ability of IL-1beta to activate an angiogenic phenotype in EI stromal cells but not in NE cells, is mediated by the IL-1 RI.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Endometriosis Endometriosis Endometrium Endothelial Growth Factors Interleukin-1 Interleukin-6 Lymphokines Adult Adult Angiogenesis Inducing Agents Angiogenesis Inducing Agents Angiogenesis Inducing Agents Animals Animals Cattle Cattle Cells, Cultured Cells, Cultured Endometrium

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