Immunolocalization and Regulation of the Chemokine RANTES in Human Endometrial and Endometriosis Tissues and Cells1

D Hornung, Daniela Hornung, I P Ryan, Isabelle P. Ryan, Victor A. Chao, V A Chao, Jean‐Louis Vigne, J L Vigne, E D Schriock, Eldon D. Schriock, R N Taylor, Robert N. Taylor
The Journal of clinical endocrinology and metabolism · 1997 · vol. 82(5) , pp. 1621–1628 · doi:10.1210/jcem.82.5.3919 · PMID:9141560 · W2006200145
article OA: bronze CC0 ⤵ 122 in-corpus citations
📄 Open PDF View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by claude@2026-06, 2026-06-06 ⓘ

This study identified RANTES mRNA and protein in human endometrium and endometriosis, demonstrating that inflammatory cytokines induce RANTES secretion by stromal cells, which likely recruits leukocytes to ectopic lesions.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

Retrograde menstruation is postulated as the initiating event in the histogenesis of endometriosis; however, subsequent steps in the pathogenesis of this common disorder remain poorly characterized. The ip accumulation of activated leukocytes and the infiltration of endometriosis lesions by macrophages and T cells are cytological markers of the inflammatory nature of this syndrome. The apparent recruitment of these leukocytes prompted us to search for chemokine expression by endometriosis cells. We reported previously that pelvic fluid RANTES (regulated upon activation, normal T cell expressed and secreted) concentrations correlated with the stage of endometriosis. In the current study, RANTES messenger ribonucleic acid (mRNA) was identified in normal endometrium and endometriosis lesions, and techniques were developed to localize RANTES protein within these tissues. Using isolated endometrial and endometriosis cell cultures, we demonstrated that RANTES mRNA and protein can be induced by the proinflammatory cytokines tumor necrosis factor-alpha and interferon-gamma in endometrial stromal, but not in epithelial or adenocarcinoma cells. Immunocytochemical studies confirmed the biochemical findings. Metabolic labeling experiments verified that nascent RANTES secreted by cytokine-stimulated endometriosis stromal cells was the mature, 8-kDa protein predicted by the mRNA encoding this chemokine. The results indicate that RANTES is a normal constituent of the eutopic endometrium. We propose that secretion of RANTES by ectopic endometriosis implants provides a mechanism for peritoneal leukocyte recruitment.

My notes (saved in your browser only)

Condition tags

endometriosis

MeSH descriptors

Chemokine CCL5 Chemokine CCL5 Endometriosis Endometrium Endometrium Gene Expression Regulation Adult Cells, Cultured Chemokine CCL5 Endometriosis Endometrium Epithelium Epithelium Female Humans Immunohistochemistry Immunosorbent Techniques RNA, Messenger RNA, Messenger RNA, Messenger

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (34)

Cited by (50)

Source provenance

europepmc
last seen: 2026-06-22T06:15:23.361955+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
pubmed
last seen: 2026-05-13T22:10:52.568893+00:00
License: CC0 · commercial use OK