Chemokine expression profiles of ovarian endometriotic stromal cells in three-dimensional culture

Ruofei Zhu; Zhu R; Nasu K; Kaei Nasu; Aoyagi Y; Yoko Aoyagi; Hirakawa T; Tomoko Hirakawa; Takebayashi K; Kanetoshi Takebayashi; Hisashi Narahara; Narahara H

doi:10.1016/j.jri.2020.103100

Chemokine expression profiles of ovarian endometriotic stromal cells in three-dimensional culture

Ruofei Zhu, Zhu R, Nasu K, Kaei Nasu, Aoyagi Y, Yoko Aoyagi, Hirakawa T, Tomoko Hirakawa, Takebayashi K, Kanetoshi Takebayashi, Hisashi Narahara, Narahara H

Journal of Reproductive Immunology · 2020 · vol. 138 , pp. 103100 · doi:10.1016/j.jri.2020.103100 · PMID:32058866 · W3004871425

article OA: closed CC0 ⤵ 11 in-corpus citations

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Three-dimensional culture of endometriotic stromal cells significantly upregulated mRNA and protein levels of several chemokines (CXCL1, CXCL2, CXCL3, CXCL8, and CCL20) compared to two-dimensional culture.

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Abstract

Endometriosis is a chronic inflammatory disease which is associated with aberrant chemokine expression. We have established a three-dimensional (3D) floating collagen gel culture of human endometriotic cyst stromal cells (ECSCs) as an in vitro model of early-stage ﬁbrosis formation in endometriosis. We evaluated the gene expression profiles of 3D-cultured ECSCs using a gene expression microarray. We identified and confirmed with reverse transcription-polymerase chain reaction that mRNA levels of CXCL1, CXCL2, CXCL3, CXCL8, and CCL20 in 3D-cultured ECSCs were significantly higher than in 2D-cultured ECSCs. The protein levels of CXCL1, CXCL2, CXCL8, and CCL20 in the supernatant of 3D-cultured ECSCs were significantly higher than in 2D-cultured ECSCs. It has been suggested that the 3D-culture model of ECSCs is more suitable for in vitro endometriosis research than 2D-culture. This microarray data provides a new platform to identify the candidate genes involved in the pathogenesis of endometriosis which could be masked in conventional 2D-culture.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Endometrium Gene Expression Regulation Primary Cell Culture Stromal Cells Adult Cells, Cultured Chemokine CCL20 Chemokine CCL20 Chemokines, CXC Chemokines, CXC Endometriosis Endometriosis Endometriosis Endometrium Endometrium Endometrium Female Fibrosis Gene Expression Profiling

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