Regulated on Activation, Normal T-Cell-Expressed and -Secreted mRNA Expression in Normal Endometrium and Endometriotic Implants

D Hornung, Daniela Hornung, Hornung D, Karin Klingel, Klingel K, K Klingel, K Dohrn, Dohrn K, Kathrin Dohrn, R Kandolf, Kandolf R, Reinhard Kandolf, Wallwiener D, D Wallwiener, Robert N. Taylor, Taylor RN, R N Taylor
The American journal of pathology · 2001 · vol. 158(6) , pp. 1949–1954 · doi:10.1016/s0002-9440(10)64664-0 · PMID:11395370 · PMC1891998 · W1737583080
article OA: bronze CC0 ⤵ 37 in-corpus citations
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This paper quantifies the mRNA expression of Regulated on Activation, Normal T-Cell-Expressed and -Secreted (RANTES) in healthy endometrial tissue and endometriosis lesions.

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Abstract

Chemoattraction of macrophages and T cells into the normal endometrium and inflammatory sites within endometriotic foci is mediated by chemokine gene expression. mRNA transcripts encoding regulated on activation, normal T-cell-expressed and -secreted (RANTES), a monocyte and T-cell chemokine, were demonstrated in the stroma of normal endometrium and endometriotic implants using in situ mRNA hybridization. Epithelial glands failed to express RANTES mRNA. In histological serial sections, we observed CD68-positive macrophages in the stroma of endometriotic implants adjacent to regions with prominent RANTES mRNA hybridization. In adjacent sections, monoclonal antibodies against tumor necrosis factor (TNF)-alpha showed this cytokine to be localized to stromal and epithelial compartments of the endometriotic implant with weak staining in unaffected ovarian tissue. Subconfluent monolayers of endometriotic stromal cells were tested for RANTES gene expression in situ, but we could only detect RANTES mRNA in isolated stromal cells after treatment with TNF-alpha. No RANTES mRNA was observed in unstimulated stromal cells or TNF-alpha stimulated or unstimulated epithelial cells. The data are consistent with a model in which proinflammatory cytokines (eg, TNF-alpha) induce RANTES gene expression limited to specific cells within endometrial and endometriotic stroma. Production of this chemokine, in turn, stimulates recruitment of CD68-positive macrophages into these tissues.

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Condition tags

endometriosis

MeSH descriptors

Chemokine CCL5 Endometriosis Endometrium Antigens, CD Antigens, CD Antigens, Differentiation, Myelomonocytic Antigens, Differentiation, Myelomonocytic Autocrine Communication CD68 Molecule Cells, Cultured Chemokine CCL5 Chemokine CCL5 Chemokine CCL5 Endometriosis Endometrium Epithelial Cells Epithelial Cells Epithelial Cells Female Humans

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License: CC0 · commercial use OK