Co‐culture with macrophages enhances the clonogenic and invasion activity of endometriotic stromal cells

Rachel Chan, Rachel W S Chan, Cheuk‐Lun Lee, Cheuk-Lun Lee, Ernest H Y Ng, Ernest Hung Yu Ng, William S B Yeung
Cell proliferation · 2017 · vol. 50(3) · doi:10.1111/cpr.12330 · PMID:28071833 · PMC6529078 · W2569865289
article OA: bronze CC0 ⤵ 32 in-corpus citations
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Co-culture with macrophages significantly increased the clonogenic and invasion ability of endometriotic stromal cells, with CSF-1 upregulated in endometriotic macrophages.

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Abstract

OBJECTIVE: To study the effect on endometrial and endometriotic cells after co-culture with macrophages, using clonogenic, invasion and self-renewal assays. MATERIALS AND METHODS: Peripheral blood samples, endometrium and endometriotic tissues were collected. Autologous macrophages were co-cultured with endometrial and endometriotic cells. The number of colony-forming units (CFU), invasiveness and self-renewal activity after co-culture with macrophages were determined. The cytokine level of colony-stimulating factor-1 (CSF-1) from macrophages with and without endometriosis was compared. RESULTS: Co-culture with macrophages significantly increased the clonogenic and invasion ability of endometriotic stromal cells in vitro. Colony-stimulating factor-1 (CSF-1) was up-regulated in endometriotic macrophages conditioned medium when compared to those without the disease. CONCLUSIONS: These data suggest that macrophages may increase the proliferation and invasion activity of stromal clonogenic cells in women with endometriosis.

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Condition tags

endometriosis

MeSH descriptors

Cell Movement Clone Cells Coculture Techniques Endometrium Macrophages Stromal Cells Adult Clone Cells Endometrium Female Humans Macrophages Middle Aged Stromal Cells

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

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Cited by (32)

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