Cytokine and immune cell levels in peritoneal fluid and peripheral blood of women with early- and late-staged endometriosis

Hikmet Hassa, Hassa H, H Hassa, Tanir Hm, H. Mete Tanir, Tekin B, B. Tekin, Kirilmaz SD, S. D. Kirilmaz, Sahin Mutlu F, Fezan Mutlu, F Sahin Mutlu
Archives of gynecology and obstetrics · 2008 · vol. 279(6) , pp. 891–895 · doi:10.1007/s00404-008-0844-8 · PMID:19023580 · W2074699010
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⚙ AI-generated summary by claude@2026-06+body, 2026-06-15 ⓘ

This study investigated cytokine and immune cell levels in peritoneal fluid and peripheral blood across different stages of endometriosis but found no significant differences between groups.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-15 · read from full text ⓘ

This prospective study compared cytokine levels and immune cell subsets in peripheral blood and peritoneal fluid from 97 women, including histopathologically confirmed endometriosis cases categorized by rASRM stage (early I/II vs late III/IV) and a control group without endometriosis. Peripheral blood serum was analyzed by ELISA for IL-2, IL-4, IL-10, and IFN-γ, while peritoneal fluid underwent the same cytokine measurements; immune phenotyping used multiparameter flow cytometry with markers for T cell subsets, NK cells, B cells, and activation antigens (e.g., CD-69 and HLA-DR). The study found no significant differences in cytokine concentrations or immune cell levels between controls and early- or late-staged endometriosis groups (p > 0.05). The paper’s main limitation is its null results for measured cytokines and lymphocyte subsets despite using relatively specific immune markers and single pre-surgery sampling. This paper is centrally about endometriosis — it directly measures cytokines and immune cell profiles in peritoneal fluid and peripheral blood across early- versus late-staged disease.

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Abstract

ObjectiveTo investigate the level of cytokines and immune cells in the peripheral blood (PB) and peritoneal fluid (PF) of different stages of endometriosis.MethodsA prospective study was conducted to include 97 women with (n 60) and without (n 37) histopathologically confirmed endometriosis. Based on rASRM classification, stage I/II and stage III/IV were categorized as early-and late-staged endometriosis. Prior to surgery, 10 ml of blood was withdrawn from antecubital vein and serum was obtained. Aliquots were made and stored at -70 degrees C until assayed for cytokines. PF was aspirated from the pouch of Douglas. Peripheral and PF samples were analyzed by ELISA in terms of IL-2, IL-4, IL-10 and IFN-gamma. Determinations of T helper, T suppressor, NK, and B cells were assessed by using cluster determinant-3 (CD-3), CD4, CD8, CD25, CD28, CD45, CD16, CD23 and antibodies against early T cell activation antigens such as CD45RA/CD45RO, CD-69 and late activation antigens such as HLA-DR. A multiparameter flow cytometry was applied to detect the cell activation antigen expression.ResultsIn terms of cytokine levels in PB and PF's of control group and early- and late-staged endometriosis cases, no significant difference was depicted in the cytokine levels (p > 0.05). Levels of immune cells did not differ between three groups (p > 0.05).ConclusionsThe result of this study did not show any significant difference in PB and PF cytokine and lymphocyte subgroups between normal and early- and late-staged endometriosis.
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Abstract

Objective To investigate the level of cytokines and immune cells in the peripheral blood (PB) and peritoneal fluid (PF) of different stages of endometriosis.

Methods

A prospective study was conducted to include 97 women with (n 60) and without (n 37) histopathologically confirmed endometriosis. Based on rASRM classification, stage I/II and stage III/IV were categorized as early-and late-staged endometriosis. Prior to surgery, 10 ml of blood was withdrawn from antecubital vein and serum was obtained. Aliquots were made and stored at −70°C until assayed for cytokines. PF was aspirated from the pouch of Douglas. Peripheral and PF samples were analyzed by ELISA in terms of IL-2, IL-4, IL-10 and IFN-γ. Determinations of T helper, T suppressor, NK, and B cells were assessed by using cluster determinant-3 (CD-3), CD4, CD8, CD25, CD28, CD45, CD16, CD23 and antibodies against early T cell activation antigens such as CD45RA/CD45RO, CD-69 and late activation antigens such as HLA-DR. A multiparameter flow cytometry was applied to detect the cell activation antigen expression.

Results

In terms of cytokine levels in PB and PF’s of control group and early- and late-staged endometriosis cases, no significant difference was depicted in the cytokine levels (p > 0.05). Levels of immune cells did not differ between three groups (p > 0.05).

Conclusions

The result of this study did not show any significant difference in PB and PF cytokine and lymphocyte subgroups between normal and early- and late-staged endometriosis. Similar content being viewed by others

References

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Arch Gynecol Obstet 265:40–44. doi:10.1007/s004040000126 Lin YJ, Lai MD, Lei HY, Wing LY (2006) Neutrophils and macrophages promote angiogenesis in the early stage of endometriosis in a mouse model. Endocrinology 147(3):1278–1286. doi:10.1210/en.2005-0790 Conflict of interest statement All the authors had conflict of interest (financial or otherwise). Author information Authors and Affiliations Corresponding author Rights and permissions About this article Cite this article Hassa, H., Tanir, H.M., Tekin, B. et al. Cytokine and immune cell levels in peritoneal fluid and peripheral blood of women with early- and late-staged endometriosis. Arch Gynecol Obstet 279, 891–895 (2009). https://doi.org/10.1007/s00404-008-0844-8 Received: Accepted: Published: Issue date: DOI: https://doi.org/10.1007/s00404-008-0844-8

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Condition tags

endometriosis

MeSH descriptors

Ascitic Fluid Endometriosis Interferon-gamma Interleukins Lymphocytes Adult Ascitic Fluid Ascitic Fluid Case-Control Studies Endometriosis Endometriosis Endometriosis Female Humans Interferon-gamma Interleukins Lymphocyte Count Lymphocytes Peritoneum Peritoneum

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