Influence of peritoneal fluid on the expression of angiogenic and proteolytic factors in cultures of endometrial cells from women with endometriosis

Cosín R, R. Cosín, Juan Gilabert–Estellés, J Gilabert-Estellés, Luis Ramón, Ramón LA, L A Ramón, Gómez-Lechón MJ, M J Gómez-Lechón, Gilabert J, J. Gilabert, Chirivella M, M. Chirivella, A Braza-Boïls, Aitana Braza‐Boïls, Francisco España, F España, Estellés A, A. Estellés
Human Reproduction · 2009 · vol. 25(2) , pp. 398–405 · doi:10.1093/humrep/dep419 · PMID:19945964 · W2169524641
Lab / animal OA: bronze CC0 ⤵ 36 in-corpus citations
📄 Open PDF View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-06-07 ⓘ

Endometriotic peritoneal fluid increased VEGF-A and uPA expression in endometrial cells from women with and without endometriosis, with the most significant increase observed in cells from endometriosis patients.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

BACKGROUND: Endometriosis, defined as the presence of endometrium outside the uterus, is one of the most frequent benign gynaecological diseases. It has been suggested that both endometrial and peritoneal factors, related to angiogenesis and proteolysis, can be implicated in this disease. The aim of this study was to evaluate the influence of peritoneal fluid on the expression of angiogenic and proteolytic factors in cultures of endometrial cells from women with and without endometriosis. METHODS: Endometrial cells were isolated, cultured and treated with endometriotic or normal peritoneal fluid. Vascular endothelial growth factor-A (VEGF-A), urokinase plasminogen activator (uPA), matrix metalloproteinase-3 (MMP-3) and their inhibitors including thrombospondin-1, plasminogen activator inhibitor-1 and MMP inhibitor type 1 (TIMP-1) mRNA levels were evaluated by quantitative RT-PCR, and protein levels were quantified by ELISA. RESULTS: Peritoneal fluid from women with endometriosis induced an increase in VEGF-A and uPA protein and VEGF-A mRNA and uPA mRNA levels in endometrial cell culture from women with (P < 0.01) and without endometriosis (P < 0.05). The highest levels of VEGF-A and uPA were observed in endometrial cell cultures from patients with endometriosis and treated with peritoneal fluid from women with endometriosis. CONCLUSIONS: Peritoneal fluid from women with endometriosis induced more VEGF and uPA expression in endometrial cell culture from women with endometriosis than did normal peritoneal fluid. Endometrial-peritoneal interactions increased angiogenic and proteolytic factors in endometrial cells, which could contribute to the development of endometriotic lesions.

My notes (saved in your browser only)

Condition tags

endometriosis

MeSH descriptors

Angiogenic Proteins Ascitic Fluid Endometriosis Endometrium Peptide Hydrolases Adult Adult Angiogenic Proteins Angiogenic Proteins Ascitic Fluid Ascitic Fluid Cells, Cultured Cells, Cultured Endometriosis Endometriosis Endometrium Endometrium Female Female Humans

Citation neighborhood (2-hop)

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

References (38)

Cited by (37)

Source provenance

europepmc
last seen: 2026-10-14T06:08:39.101224+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
pubmed
last seen: 2026-10-08T21:07:04.513278+00:00
License: CC0 · commercial use OK