IL-1 beta, TNF-alpha, and IL-2 in peritoneal fluid and macrophage-conditioned media of women with endometriosis

JEFFREY A. KEENAN, J A Keenan, Keenan JA, Jeffrey Keenan, T T Chen, Chen TT, Thomas T. Chen, Nancy L. Chadwell, Chadwell NL, N L Chadwell, D S Torry, Torry DS, Donald S. Torry, Caudle MR, M R Caudle, Michael R. Caudle
American journal of reproductive immunology (New York, N.Y. : 1989) · 1995 · vol. 34(6) , pp. 381–385 · doi:10.1111/j.1600-0897.1995.tb00968.x · PMID:8607944 · W2005309779
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Endometriosis patients exhibited significantly higher IL-1 beta and TNF-alpha levels in macrophage conditioned media, but not in peritoneal fluid, suggesting peritoneal macrophage activation.

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Abstract

PROBLEM: The presence of various cytokines in human peritoneal fluid has been incompletely evaluated. Changes in cytokine levels may be related to the development of endometriosis, infertility, and activation of peritoneal macrophages. This study assesses levels of IL-1 beta, IL-2 and TNF- alpha in peritoneal fluid and macrophage conditioned media of women with endometriosis. METHOD: Peritoneal fluid was collected from 51 women at the time of diagnostic or operative laparoscopy for benign gynecologic disease. Peritoneal macrophages were isolated, cultured for 24 h, and the culture media collected. IL-1 beta, IL-2, and TNF- alpha levels were determined by commercial ELISA kits. RESULTS: The mean concentration of IL-1 beta and TNF- alpha was significantly higher in macrophage conditioned media of patients with endometriosis (P < 0.02). However, there were no significant changes in peritoneal fluid cytokine levels. Peritoneal macrophage concentrations were also higher in patients with endometriosis. CONCLUSION: This study supports the concept that endometriosis is associated with activation of peritoneal macrophages, and a higher concentration of these cells. This activation is reflected by the increased levels of cytokines found in macrophage conditioned media. The absence of significant changes in peritoneal fluid cytokine levels would seen to indicate that the above derangements are not responsible for the development or progression of endometriosis.

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Condition tags

endometriosisinfertility

MeSH descriptors

Ascitic Fluid Endometriosis Interleukin-1 Interleukin-2 Macrophages Tumor Necrosis Factor-alpha Adult Adult Ascitic Fluid Ascitic Fluid Culture Media, Conditioned Culture Media, Conditioned Endometriosis Endometriosis Endometriosis Female Female Humans Humans Immunity, Cellular

Citation neighborhood (2-hop)

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

References (42)

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