A Botanical Extract from Channel Flow Inhibits Cell Proliferation, Induces Apoptosis, and Suppresses CCL5 in Human Endometriotic Stromal Cells1

Fritz Wieser, Jie Yu, John Park, Cheol Park, Andrew Gaeddert, Misha Cohen, Jean‐Louis Vigne, Jean-Louis Vigne, Robert N Taylor
Biology of reproduction · 2009 · vol. 81(2) , pp. 371–377 · doi:10.1095/biolreprod.108.075069 · PMID:19403929 · W2161421006
article OA: bronze CC0 ⤵ 22 in-corpus citations
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This study found that a botanical extract dose-dependently inhibited cell proliferation, induced apoptosis, and suppressed CCL5 gene expression and protein secretion in human endometriotic stromal cells.

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Abstract

Growing evidence suggests that medicinal herbs have direct actions on endometrial cells. By screening multiple herbs using an in vitro model of endometriosis, we found that a commonly used herbal formula exerted considerable antiproliferative effects. Our purpose was to investigate the effects of this antiendometriosis herbal mixture on cell proliferation, apoptosis, and CCL5 expression and secretion in endometriotic stromal cells in vitro. Isolated normal endometrial, eutopic, and ectopic endometriotic stromal cells were cultured under established conditions. Cell proliferation, apoptosis, and CCL5 gene expression protein secretion was evaluated after incubation with different concentrations of an antiendometriosis herbal mixture extract. Cell proliferation was assessed by cell counting, (3)H-thymidine incorporation, and MTS assays. Apoptosis was determined by blotting using anti-cleaved caspase 3 antibodies and by a TUNEL assay. CCL5 gene expression and protein secretion were determined by transient transfection of gene promoter reporters and ELISAs in cell supernatants. Extracts of a traditional herbal mixture dose-dependently decreased cell proliferation in normal, eutopic, and ectopic endometriotic stromal cells. (3)H-Thymidine uptake and MTS confirmed these findings. The herbal extracts induced apoptosis, as evidenced by activation of caspase 3 and the presence of TUNEL-positive cells after treatment. The herbal extracts also suppressed CCL5 gene transcription and protein secretion in endometriotic stromal cells, even when corrected for cell number. Extracts from a medicinal herbal mixture have direct effects on cell proliferation, apoptosis, and CCL5 production in endometriotic stromal cells. Our findings support the further investigation of novel, potentially safe and well-tolerated botanical products as future endometriosis treatments.

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Condition tags

mesh:D004715endometriosis

MeSH descriptors

Anti-Inflammatory Agents, Non-Steroidal Apoptosis Cell Proliferation Chemokine CCL5 Endometriosis Magnoliopsida Stromal Cells Analysis of Variance Anti-Inflammatory Agents, Non-Steroidal Apoptosis Caspase 3 Caspase 3 Cell Proliferation Cells, Cultured Chemokine CCL5 Chemokine CCL5 Dose-Response Relationship, Drug Drugs, Chinese Herbal Drugs, Chinese Herbal Endometriosis

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