Deficient 17beta-hydroxysteroid dehydrogenase type 2 expression in endometriosis: failure to metabolize 17beta-estradiol

K Zeitoun, Khaled Zeitoun, Zeitoun K, K Takayama, Kazuto Takayama, Takayama K, H Sasano, Sasano H, Hironobu Sasano, Suzuki T, T Suzuki, Takashi Suzuki, N Moghrabi, Moghrabi N, Nabil Moghrabi, S Andersson, Andersson S, Stefan Andersson, A Johns, Alan Johns, Johns A, Meng Li, L Meng, Meng L, M Putman, Putman M, Michael Putman, Carr B, Bruce R. Carr, B. Carr, Serdar E. Bulun, S E Bulun, Bulun Se
The Journal of clinical endocrinology and metabolism · 1998 · vol. 83(12) , pp. 4474–4480 · doi:10.1210/jcem.83.12.5301 · PMID:9851796 · W2117304335
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Endometriotic lesions showed deficient 17β-hydroxysteroid dehydrogenase type 2 expression, impairing 17β-estradiol inactivation normally induced by progesterone in eutopic endometrium.

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Abstract

Aberrant aromatase expression in stromal cells of endometriosis gives rise to conversion of circulating androstenedione to estrone in this tissue, whereas aromatase expression is absent in the eutopic endometrium. In this study, we initially demonstrated by Northern blotting transcripts of the reductive 17beta-hydroxysteroid dehydrogenase (17betaHSD) type 1, which catalyzes the conversion of estrone to 17beta-estradiol, in both eutopic endometrium and endometriosis. Thus, it follows that the product of the aromatase reaction, namely estrone, that is weakly estrogenic can be converted to the potent estrogen, 17beta-estradiol, in endometriotic tissues. It was previously demonstrated that progesterone stimulates the inactivation of 17beta-estradiol through conversion to estrone in eutopic endometrial epithelial cells. Subsequently, 17betaHSD type 2 was shown to catalyze this reaction, and its transcripts were detected in the epithelial cell component of the eutopic endometrium in secretory phase. Because 17beta-estradiol plays a critical role in the development and growth of endometriosis, we studied 17betaHSD-2 expression in endometriotic tissues and eutopic endometrium. We demonstrated, by Northern blotting, 17betaHSD-2 messenger ribonucleic acid (RNA) in all RNA samples of secretory eutopic endometrium (n=12) but not in secretory samples of endometriotic lesions (n=10), including paired samples of endometrium and endometriosis obtained simultaneously from eight patients. This messenger RNA was not detectable in any samples of proliferative eutopic endometrium or endometriosis (n=4) as expected. Next, we confirmed these findings by demonstration of immunoreactive 17betaHSD-2 in epithelial cells of secretory eutopic endometrium in 11 of 13 samples employing a monoclonal antibody against 17betaHSD-2, whereas 17betaHSD-2 was absent in paired secretory endometriotic tissues (n=4). Proliferative eutopic endometrial (n=8) and endometriotic (n=4) tissues were both negative for immunoreactive 17betaHSD-2, except for barely detectable levels in 1 eutopic endometrial sample. Finally, we sought to determine whether deficient 17betaHSD-2 expression in endometriotic tissues is due to impaired progesterone action in endometriosis. We determined by immunohistochemistry the expression of progesterone and estrogen receptors in these paired samples of secretory (n=4) and proliferative (n=4) eutopic endometrium and endometriosis, and no differences could be demonstrated. In conclusion, inactivation of 17beta-estradiol is impaired in endometriotic tissues due to deficient expression of 17betaHSD-2, which is normally expressed in eutopic endometrium in response to progesterone. The lack of 17betaHSD-2 expression in endometriosis is not due to alterations in the levels of immunoreactive progesterone or estrogen receptors in this tissue and may be related to an inhibitory aberration in the signaling pathway that regulates 17betaHSD-2 expression.

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Condition tags

endometriosis

MeSH descriptors

17-Hydroxysteroid Dehydrogenases Endometriosis Estradiol Isoenzymes 17-Hydroxysteroid Dehydrogenases 17-Hydroxysteroid Dehydrogenases 17-Hydroxysteroid Dehydrogenases Adult Adult Blotting, Northern Blotting, Northern Endometriosis Endometriosis Endometriosis Endometrium Endometrium Endometrium Endometrium Estradiol Estradiol

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References (36)

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