Soluble factors from human endometrium promote angiogenesis and regulate the endothelial cell transcriptome

Cristin Print, Cristin G. Print, Valtola R, Reija Valtola, Amanda Evans, Evans A, Lessan K, Khashayar Lessan, Malik S, Shazia Malik, S. K. Smith, Stephen Smith
Human Reproduction · 2004 · vol. 19(10) , pp. 2356–2366 · doi:10.1093/humrep/deh411 · PMID:15242995 · W2101880027
Lab / animal OA: bronze CC0 ⤵ 14 in-corpus citations
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Human endometrial supernatants, particularly from the proliferative phase, contain soluble factors including VEGF-A that promote endothelial cell proliferation, angiogenesis, and alter the endothelial transcriptome.

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Abstract

BACKGROUND: Angiogenesis and vascular remodeling play critical roles in the cyclical growth and regression of endometrium. They also appear to play roles in the pathogenesis of endometriosis. METHODS AND RESULTS: Supernatants were collected from cultured endometrium isolated from women with and without endometriosis. These supernatants induced endothelial cell proliferation and angiogenesis in vitro. They contained vascular endothelial growth factor (VEGF)-A, and their proliferative effects on endothelial cells were partially abrogated by a blocking anti-VEGF-A antibody. Gene array analysis showed that culture supernatants from proliferative phase endometrium, and to a lesser extent secretory phase endometrium, induced significant changes in the transcriptome of endothelial cells. We could not detect any association between endometriosis and the ability of endometrial-derived soluble factors to promote angiogenesis or to regulate the endothelial transcriptome. In addition, we could not detect any association between endometriosis and the concentration of VEGF-A in supernatants from cultured endometrium or in menstrual effluent. CONCLUSIONS: We have shown that endometrium cultured in vitro produced soluble factors, including VEGF-A, that promoted angiogenesis. Proliferative phase endometrium promoted significant endothelial cell transcriptome changes that appear overall to be pro-angiogenic. These transcriptome changes provide insight into the dynamic control of vessel structure on which both eutopic endometrium and endometriotic lesions depend.

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Condition tags

endometriosis

MeSH descriptors

Endometrium Endometrium Endothelial Cells Neovascularization, Physiologic Neovascularization, Physiologic Neovascularization, Physiologic Transcription, Genetic Transcription, Genetic Transcription, Genetic Case-Control Studies Case-Control Studies Cell Division Cell Division Cell Division Culture Media Culture Media Culture Media Culture Media Endometriosis Endometriosis

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References (63)

Cited by (13)

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