Interleukin-17A is present in neutrophils in endometrioma and stimulates the secretion of growth-regulated oncogene-α (Gro-α) from endometrioma stromal cells

Masashi Takamura, Takamura M, Yutaka Osuga, Osuga Y, Izumi G, Gentaro Izumi, Yoshino O, Osamu Yoshino, Kaori Koga, Ako Saito, Saito A, Tetsuya Hirata, Yasushi Hirota, Miyuki Harada, Akiko Hasegawa, Hasegawa A, Taketani Y, Yuji Taketani
Fertility and sterility · 2012 · vol. 98(5) , pp. 1218–1224.e2 · doi:10.1016/j.fertnstert.2012.07.1117 · PMID:22902060 · W2054225708
Lab / animal OA: closed CC0 ⤵ 22 in-corpus citations
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Interleukin-17A was detected in neutrophils within endometrioma tissue, where it promoted the release of Gro-α from stromal cells.

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Abstract

OBJECTIVE: To investigate a new role of interleukin (IL)-17A in endometriosis. DESIGN: Laboratory study. SETTING: University hospital. PATIENT(S): Patients with ovarian endometrioma undergoing laparoscopy or laparotomy. INTERVENTION(S): Primary culture of endometrioma stromal cells (EoSCs) was stimulated with IL-17A. Sections of endometrioma tissue were immunostained with antibodies for IL-17A, growth-regulated oncogene-α (Gro-α), and elastase, a marker of neutrophils. They were also examined with immunofluorescent double staining for IL-17A and myeloperoxidase, another marker of neutrophils. MAIN OUTCOME MEASURE(S): Concentration of Gro-α was measured using a specific ELISA. Neutrophil chemotaxis was measured with Boyden chamber method. Immunostained sections were examined under microscope. RESULT(S): Interleukin-17A increased the secretion of Gro-α from EoSCs dose-dependently. The conditioned medium of EoSCs stimulated with IL-17A attracted more neutrophils than that of EoSCs stimulated with vehicle, and the increase was inhibited by the addition of Gro-α-neutralizing antibody. On immunostaining, IL-17A and Gro-α were detected in similar areas of the stroma beneath the epithelium, where Gro-α was detected in cells with a stromal cell appearance whereas IL-17A was detected in neutrophils as determined by detection of elastase. Fluorescent immunostaining corroborated that myeloperoxidase-positive neutrophils were also positive for IL-17A. CONCLUSION(S): It is suggested that IL-17A produced by neutrophils stimulates Gro-α secretion from EoSCs, thereby recruiting more neutrophils and inducing perpetuating inflammation in endometriosis.

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Condition tags

endometriosisendometrioma

MeSH descriptors

Chemokine CXCL1 Endometriosis Endometrium Interleukin-17 Neutrophils Paracrine Communication Paracrine Communication Stromal Cells Cells, Cultured Cells, Cultured Chemokine CXCL1 Chemokine CXCL1 Chemotaxis, Leukocyte Chemotaxis, Leukocyte Culture Media, Conditioned Culture Media, Conditioned Culture Media, Conditioned Endometriosis Endometriosis Endometriosis

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Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

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