Effect of danazol on estradiol-17beta and progesterone secretion by porcine ovarian cells in vitro

Benjamin K. Tsang, B K Tsang, K. M. Henderson, David T. Armstrong, D T Armstrong
American journal of obstetrics and gynecology · 1979 · vol. 133(3) , pp. 256–259 · doi:10.1016/0002-9378(79)90675-6 · PMID:433984 · W2242473744
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Danazol inhibited both basal and LH-stimulated progesterone secretion by porcine granulosa and luteal cells, and testosterone-stimulated estradiol secretion by granulosa cells in vitro.

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Abstract

The effect of danazol on progesterone (P4) and estradiol-17 beta (E2) secretion by cultured porcine granulosa cells was studied. Danazol markedly inhibited both basal and luteinizing hormone (LH)-stimulated P4 secretion by both granulosa and luteal cells in a dose-dependent manner during 24 hour culture periods. E2 secretion by granulosa cells was low (less than 3 ng/mg of protein) when cultured in the absence of an exogenous aromatizable substrate but was markedly increased when testosterone (T) was added to the media. Danazol inhibited E2 secretion by granulosa cells only when cultured in the presence of testosterone. E2 secretion by luteal cells was low even in the presence of testosterone and was not affected by danazol. These findings support the hypothesis that danazol can directly inhibit ovarian steroidogenesis independently of its inhibitory action on gonadotropin secretion.

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MeSH descriptors

Danazol Estradiol Granulosa Cells Pregnadienes Progesterone Animals Cells, Cultured Culture Media Danazol Dose-Response Relationship, Drug Estradiol Female Granulosa Cells Granulosa Cells Luteal Cells Luteal Cells Luteal Cells Luteinizing Hormone Luteinizing Hormone Pregnadienes

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