Inhibitory effects of danazol on steroidogenesis in cultured human granulosa cells

Jan‐Henrik Olsson, J H Olsson, T Hillensjö, Torbjörn Hillensjø, Lars Nilsson, L Nilsson
Fertility and sterility · 1986 · vol. 46(2) , pp. 237–242 · doi:10.1016/s0015-0282(16)49518-x · PMID:3732530 · W2417307952
Lab / animal OA: closed CC0 ⤵ 11 in-corpus citations
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Danazol treatment significantly suppressed testosterone and progesterone production in cultured human granulosa cells.

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Abstract

The effect of danazol on steroidogenesis in cultured human granulosa cells was studied. Granulosa cells obtained from antral follicles in the mid to late follicular phase were cultured for 2 to 6 days. Danazol (0.1 to 5 micrograms/ml) and human chorionic gonadotropin (hCG, 10 IU/ml), alone or in combination, were added to the culture medium. Testosterone (T) (1 microgram/ml) was added as an aromatase substrate in certain experiments. The medium content of progesterone (P) and estradiol (E2) was determined. Basal P secretion was variably influenced by danazol, whereas a consistent dose-dependent and reversible inhibition of the hCG-stimulated P secretion was found. In the presence of T, danazol caused a dose-dependent inhibition of both basal and hCG-stimulated E2 secretion, and this effect became more pronounced with time. The results demonstrate that danazol exerts direct inhibitory effects on steroidogenesis in cultured human granulosa cells.

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MeSH descriptors

Danazol Granulosa Cells Pregnadienes Steroids Adult Animals Chorionic Gonadotropin Chorionic Gonadotropin Cricetinae Danazol Dose-Response Relationship, Drug Estradiol Estradiol Female Granulosa Cells Humans Middle Aged Pregnadienes Progesterone Progesterone

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