Lipopolysaccharide-promoted proliferation of endometriotic stromal cells via induction of tumor necrosis factor alpha and interleukin-8 expression

Iba Y, Yumiko Iba, Tasuku Harada, Harada T, Sayako Horie, Horie S, Deura I, Imari Deura, Tomio Iwabe, Iwabe T, Naoki Terakawa, Terakawa N
Fertility and sterility · 2004 · vol. 82 , pp. 1036–1042 · doi:10.1016/j.fertnstert.2004.04.038 · PMID:15474070 · W2061150039
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Lipopolysaccharide stimulates endometriotic stromal cell proliferation by upregulating tumor necrosis factor α and interleukin-8 expression.

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Abstract

OBJECTIVE: To evaluate the effect of lipopolysaccharide (LPS) on the expression of tumor necrosis factor alpha (TNFalpha) and interleukin-8 (IL-8) protein in endometriotic stromal cells (ESC) and their effect on the proliferation of ESC. DESIGN: Prospective study. SETTING: Department of Obstetrics and Gynecology, Tottori University Hospital, Yonago, Japan. PATIENT(S): Seventeen patients who underwent laparoscopic surgery. INTERVENTION(S): Endometriotic stromal cells were obtained from chocolate cyst linings of the ovary. MAIN OUTCOME MEASURE(S): We determined the effect of LPS on the production of TNFalpha and IL-8 and the effect of IL-8 antisense oligonucleotide and nuclear factor-kappaB (NF-kappaB) inhibitor on IL-8 production using ELISA. TNFalpha production was examined by immunocytochemical staining. We determined the effect of LPS and the effect of IL-8 antisense oligonucleotide and NF-kappaB inhibitor on LPS-promoted ESC proliferation. RESULT(S): LPS-stimulated ESC produced significant amounts of TNFalpha and IL-8 in a dose- and time-dependent fashion. Adding LPS promoted ESC proliferation. Anti-TNFalpha antibody and anti-IL-8 antibody inhibited the stimulatory effects of LPS. IL-8 antisense oligonucleotide and NF-kappaB inhibitor significantly decreased LPS-induced IL-8 protein production and LPS-induced ESC proliferation. CONCLUSION(S): Pelvic inflammation may promote the progression of endometriosis.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Interleukin-8 Lipopolysaccharides Stromal Cells Tumor Necrosis Factor-alpha Cell Division Cell Division Cell Division Dose-Response Relationship, Drug Dose-Response Relationship, Drug Endometriosis Endometriosis Endometriosis Female Female Gene Expression Gene Expression Humans Humans Immunohistochemistry

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