Epidermal growth factor in human endometrium: proliferative effects in culture and immunocytochemical localization in normal and endometriotic tissues
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⤵ 37 in-corpus citations
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This study found that epidermal growth factor (EGF) combined with oestradiol stimulates glandular endometrial proliferation in culture, and EGF is present in both normal and endometriotic tissues.
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Abstract
Endometrial proliferation is stimulated by oestradiol. The precise mechanism is poorly understood, but may be mediated by epidermal growth factor (EGF). The aim of the present study was to assess the effects of oestradiol and EGF on glandular and stromal proliferation in human endometrial cell cultures, and to determine the localization of EGF-like immunoreactivity (EGF-IR) using immunocytochemistry in normal and endometriotic tissue. Endometrium was obtained from women undergoing curettage or hysterectomy for benign disease, or laparoscopy for endometriosis. For tissue culture experiments, enriched glandular and stromal cells were prepared by digestion with collagenase and DNAase, and cultured for 4 days with oestradiol or EGF, both alone and in combination. Immunocytochemical studies were performed using sheep primary antibody against EGF, with binding visualized using the unlabelled antibody--enzyme method. In combination, oestradiol and EGF increased mean cell counts from 1.15 +/- 0.06 and 1.36 +/- 0.05 x 10(5)/ml to 1.68 +/- 0.1 (+46%) and 1.94 +/- 0.16 (+43%) x 10(5)/ml, in proliferative and secretory gland preparations respectively (n = 10, P less than 0.01). No effect was seen in stromal cell preparations; however the stimulation in gland preparations was further augmented after the addition of stromal-conditioned medium. EGF-IR was detected in endometrium from normal women, and in normal and ectopic endometrium from women with endometriosis. EGF-IR was seen in glands and stroma and was not related to the phase of the menstrual cycle. EGF may play a role in the oestrogen-stimulated proliferation of normal and endometriotic endometrium.
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Cited by (38)
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- An Investigation Into the Mechanism of Li Chong Pill for Treating Endometriosis Based on Network Pharmacology and Molecular Docking Verification 2021
- MAP kinases and the inflammatory signaling cascade as targets for the treatment of endometriosis? 2015
- Macrophages in Pathophysiology of Endometriosis 2014
- Roles of Prostaglandin E2 in Endometriosis 2014
- Peritoneal, Ovarian, and Rectovaginal Endometriosis are Three Different Entities 2011
- The role of prostaglandin E2 in endometriosis 2011
- The Mechanism of Menstruation 2010
- Prostaglandin E2: the master of endometriosis? 2010
- Prostaglandin E2: the master of endometriosis? 2010
- Expression and Role of Peptides, Proteins and Growth Factors in the Pathogenesis of Endometriosis 2009
- Expression and role of peptides, proteins and growth factors in the pathogenesis of endometriosis 2009
- Endometriosis: disease pathophysiology and the role of prostaglandins 2007
- Prostaglandin E2 induces fibroblast growth factor 9 via EP3-dependent protein kinase Cdelta and Elk-1 signaling 2006
- In vivo assessment of the regulation of transforming growth factor alpha, epidermal growth factor (EGF), and EGF receptor in the human endometrium by medroxyprogesterone acetate 2005
- Expression and mitogenic effect of fibroblast growth factor-9 in human endometriotic implant is regulated by aberrant production of estrogen 2003
- Endometrial anomalies in women with endometriosis 2001
- Epidermal growth factor regulates the transition from basal sodium absorption to anion secretion in cultured endometrial epithelial cells 2001
- The concept of ‘adenomyotic disease of the retroperitoneal space’ is born 2001
- Reduced proliferation and cell adhesion in endometriosis 2000
- Endometrial-myometrial interface: relationship to adenomyosis and changes in pregnancy 2000
- Quantitative enzyme immunoassay and semiquantitative immunohistochemistry of oestrogen and progesterone receptors in endometriotic tissue and endometrium 1997
- Immunohistochemical analysis of insulin-like growth factor I, insulin-like growth factor I receptor and insulin-like growth factor II in endometriotic tissue and endometrium 1997
- Peritoneal endometriosis, ovarian endometriosis, and adenomyotic nodules of the rectovaginal septum are three different entities 1997
- Immunology and endometriosis. Fact, artifact, or epiphenomenon? 1997
- Basic research in endometriosis 1997
- Flow cytometric DNA analysis in endometriotic tissue compared to normal uterine endometrium 1996
- Prostaglandins and menstruation 1996
- A comparison of cathepsin D levels in endometriotic tissue and in uterine endometrium 1996
- A comparison of cathepsin D levels in endometriotic tissue and in uterine endometrium 1996
- Growth factors and growth modulators in human uterine endometrium: their potential relevance to reproductive medicine 1994
- The actions of estradiol and epidermal growth factor in endometrial and endometriotic stroma in vitro 1994
- Endometriosis: still an enigma 1993
- Heparin-sepharose binding growth factors in peritoneal fluid of women with endometriosis 1993
- Heparin-sepharose binding growth factors in peritoneal fluid of women with endometriosis 1993
- Angiogenic activity of peritoneal fluid from women with endometriosis 1993
- Prostaglandins and Menstruation 1992
- The aetiology and pathogenesis of endometriosis 1992
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