Heparin-sepharose binding growth factors in peritoneal fluid of women with endometriosis
article
OA: closed
CC0
⤵ 17 in-corpus citations
Abstract
OBJECTIVE: To characterize and to purify the growth substances for mouse embryo fibroblasts (NIH/3T3) and endometrial-like cells of the peritoneal fluid of women with endometriosis. DESIGN: A pool of 102 mL of peritoneal fluid (PF) collected from 24 women with laparoscopic evidence of endometriosis was dialyzed and thereafter chromatographed onto carboxymethyl (CM)-sepharose, heparin-sepharose, cartridges of C18 silica, and reverse-phase high-performance liquid chromatography (reverse-phase HPLC). Mitogenic activity throughout various steps of chromatography was monitored by the increase of the (3H)-thymidine incorporation into NIH/3T3 fibroblasts and enzymatically isolated epithelial cells of rabbit endometrium. RESULTS: Peritoneal fluid of women with endometriosis contained mitogen(s) for NIH/3T3 fibroblasts that were retained on and eluted from CM-sepharose, heparin-sepharose, cartridges of C18 silica, and reverse-phase HPLC columns performed sequentially. Mitogenic material purified 2,500-fold exerted bioactivity on NIH/3T3 and the enzymatically isolated epithelial cells from rabbit endometrium. CONCLUSION: Peritoneal fluid of women with endometriosis contains heparin-binding growth factors that can be purified by sequential chromatography on CM-sepharose, heparin-sepharose, and reverse-phase HPLC columns. The presence in the PF of potent mitogens for fibroblasts and endometrium-derived epithelial cells could play an important role in the pathogenesis of endometriosis.
My notes (saved in your browser only)
Condition tags
MeSH descriptors
Citation neighborhood
Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.
References (18)
- Deficient cellular immunity in endometriosis via openalex
- Endometriosis: Role of Ovarian Steroids in Initiation, Maintenance, and Suppression via openalex
- Epidermal growth factor in human endometrium: proliferative effects in culture and immunocytochemical localization in normal and endometriotic tissues via openalex
- Peritoneal fluid volume, estrogen, progesterone, prostaglandin, and epidermal growth factor concentrations in patients with and without endometriosis via openalex
- W2039144212 via openalex
- W2046373576 via openalex
- W2060749467 via openalex
- W2065217183 via openalex
- W2094008116 via openalex
- W2100837269 via openalex
- W2103824972 via openalex
- W2114060808 via openalex
- W2172013394 via openalex
- W141728048 via openalex
- W2419401254 via openalex
- W1999646634 via openalex
- W2004819339 via openalex
- W2005247421 via openalex
Cited by (17)
- Glucocorticoid receptor function suppresses insulin-like growth factor 1 activity in human KLE endometrial-like cells. 2004
- Urokinase‐Type Plasminogen Activator and Insulin‐Like Growth Factor‐Binding Protein 3 mRNA Expression in Endometriotic Lesions and Eutopic Endometrium 2003
- Macrophages, Oxidation, and Endometriosis 2002
- Identification of an Invasive, N-Cadherin-Expressing Epithelial Cell Type in Endometriosis Using a New Cell Culture Model 2001
- Increased Insulin-like Growth Factor 1 Activity Can Rescue KLE Endometrial-like Cells from Apoptosis 2001
- Immunohistochemical localization of insulin-like growth factor–binding protein-3 in eutopic and ectopic endometrial tissues 1999
- Role of Cytokines in Progression of Endometriosis 1999
- Pathogenesis of Endometriosis: The Role of Peritoneal Fluid 1999
- IGFs in the Female Reproductive System 1999
- Endometriotic disease: the role of peritoneal fluid 1998
- Tracing cellular and molecular mechanisms involved in endometriosis 1998
- Atypical Endometriosis: Its Presentation and Management 1998
- Growth factors and cytokines in the reproductive tract of women 1997
- Epidermal growth factor and basic fibroblast growth factor in peritoneal fluid of women with endometriosis 1996
- Pain relief after combined medical and laparoscopic conservative treatment of stage III–IV endometriosis: A comparison with medical therapy 1996
- N-terminal truncated forms of insulin-like growth factor binding protein-3 in the peritoneal fluid of women without laparoscopic evidence of endometriosis 1995
- The insulin-like growth factor system in human peritoneal fluid: its effects on endometrial stromal cells and its potential relevance to endometriosis. 1994
Source provenance
- europepmc
- last seen: 2026-06-04T01:30:01.192114+00:00
- openalex
- last seen: 2026-06-04T00:00:01.174412+00:00
- pubmed
- last seen: 2026-05-13T22:11:44.647872+00:00
License: CC0
· commercial use OK