The actions of estradiol and epidermal growth factor in endometrial and endometriotic stroma in vitro

Mellor SJ, Sally Jane Mellor, S J Mellor, E J Thomas, Eric J. Thomas, Thomas EJ
Fertility and sterility · 1994 · vol. 62(3) , pp. 507–513 · doi:10.1016/s0015-0282(16)56938-6 · PMID:8062945 · W2408803317
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Estradiol and epidermal growth factor were found to promote proliferation and inhibit apoptosis in human endometrial and endometriotic stromal cells in vitro.

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Abstract

ObjectivesTo compare the effects of E2 and epidermal growth factor (EGF) on DNA and protein synthesis in endometrial and endometriotic stromal cell culture.DesignMeasurement of DNA and protein synthesis in cell cultures by trichloroacetic acid precipitation of radioactively labeled macromolecules.SettingTissue samples collected in operating theaters were processed in the cell culture laboratory.PatientsSamples of endometrium and endometriosis were collected synchronously at laparotomy.InterventionsStromal cells were isolated from tissue samples. Cultures of endometrial and endometriotic stromal cells were treated with 0.272 ng/mL (1 nmol/L) E2 or 10 ng/mL (1.6 nmol/L) EGF.Main outcome measures3H-Thymidine and 3H-leucine incorporation were used to assess DNA and protein synthesis respectively in cell cultures.ResultsThere was no response to 0.272 ng/mL (1 nmol/L) E2 with respect to DNA or protein synthesis in either endometrial or endometriotic stromal cells, whereas 10 ng/mL (1.6 nmol/L) EGF significantly stimulated DNA and protein synthesis in both cell types. The effect of EGF was significantly greater in endometrial stroma than in endometriotic stroma.ConclusionsThere are differences in the stimulation of 3H-thymidine incorporation by 10 ng/mL (1.6 nmol/L) EGF in endometrial and endometriotic stromal cells in vitro.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Endometrium Epidermal Growth Factor Estradiol Stromal Cells Cells, Cultured DNA DNA Endometriosis Endometriosis Endometrium Endometrium Endometrium Epidermal Growth Factor Estradiol Female Humans Leucine Leucine Protein Biosynthesis

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