Dose–response effect of interleukin (IL)-1β, tumour necrosis factor (TNF)-α, and interferon-γ on the in vitro production of epithelial neutrophil activating peptide-78 (ENA-78), IL-8, and IL-6 by human endometrial stromal cells

Nick A Bersinger, Andreas R Günthert, Brett McKinnon, Silke Johann, Michael D Mueller
Archives of gynecology and obstetrics · 2010 · vol. 283(6) , pp. 1291–1296 · doi:10.1007/s00404-010-1520-3 · PMID:20505949 · W2017195223
article OA: bronze CC0 ⤵ 29 in-corpus citations
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⚙ AI-generated summary by claude@2026-06+body, 2026-06-09 ⓘ

This study analyzed the dose-dependent production of ENA-78, IL-6, and IL-8 by human endometrial stromal cells stimulated by IL-1β, TNF-α, and interferon-γ.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-09 ⓘ

The study examined how different doses of IL-1β, TNF-α, and interferon-γ affect in vitro production of the neutrophil-activating chemokine ENA-78 and the cytokines IL-8 and IL-6 by human endometrial stromal cells. Stromal cells derived from eutopic endometrium of seven cycling endometriosis-free women were isolated and cultured (including monolayers) and then exposed to IL-1β (0.08–50 ng/mL), TNF-α, and interferon-γ (20–500 ng/mL), with ENA-78, IL-8, and IL-6 release measured. IL-1β increased all three mediators dose-dependently but showed decreased IL-8 and IL-6 release at the highest IL-1β dose tested, while TNF-α produced a plateau effect across a dose range and interferon-γ had opposite effects by inhibiting IL-8 above 20 ng/mL while stimulating IL-6 and largely leaving ENA-78 unaffected. Limitations include small sample size (n=7) and reliance on eutopic, endometriosis-free stromal cells rather than endometriotic tissue. This paper is centrally about endometriosis in its use of IL-8 and ENA-78 biology, explicitly noting that IL-8 and ENA-78 are increased in endometriosis peritoneal fluid and framing stromal cytokine regulation relevant to endometriosis.

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MeSH descriptors

Chemokine CXCL5 Endometrium Endometrium Interferon-gamma Interleukin-1beta Interleukin-6 Interleukin-8 Stromal Cells Stromal Cells Tumor Necrosis Factor-alpha Cells, Cultured Chemokine CXCL5 Dose-Response Relationship, Drug Endometrium Endometrium Female Humans Interferon-gamma Interleukin-1beta Interleukin-6

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