Selective Inhibition of Prostaglandin E2 Receptors EP2 and EP4 Inhibits Adhesion of Human Endometriotic Epithelial and Stromal Cells Through Suppression of Integrin-Mediated Mechanisms1

JeHoon Lee, Sakhila K. Banu, Banu SK, Robert C. Burghardt, Starzinski-Powitz A, Anna Starzinski‐Powitz, Anna Starzinski-Powitz, Arosh JA, Joe A. Arosh
Biology of reproduction · 2012 · vol. 88(3) , pp. 77 · doi:10.1095/biolreprod.112.100883 · PMID:23242524 · PMC4013861 · W2105208832
article OA: bronze CC0 ⤵ 23 in-corpus citations
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Selective inhibition of prostaglandin E2 receptors EP2 and EP4 suppressed integrin subunit expression and signaling, reducing endometriotic cell adhesion to extracellular matrix proteins.

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Abstract

Endometriosis is a chronic gynecological disease of reproductive age women characterized by the presence of functional endometrial tissues outside the uterine cavity. Interactions between the endometriotic cells and the peritoneal extracellular matrix proteins (ECM) are crucial mechanisms that allow adhesion of the endometriotic cells into peritoneal mesothelia. Prostaglandin E2 (PGE2) plays an important role in the pathogenesis of endometriosis. In previous studies, we have reported that selective inhibition of PGE2 receptors PTGER2 and PTGER4 decreases survival and invasion of human endometriotic epithelial and stromal cells through multiple mechanisms. Results of the present study indicates that selective inhibition of PTGER2- and PTGER4-mediated PGE2 signaling 1) decreases the expression and/or activity of specific integrin receptor subunits Itgb1 (beta1) and Itgb3 (beta3) but not Itgb5 (beta5), Itga1 (alpha1), Itga2 (alpha2), Itga5 (alpha5), and Itgav (alphav); 2) decreases integrin-signaling components focal adhesion kinase or protein kinase 2 (PTK2) and talin proteins; 3) inhibits interactions between Itgb1/Itgb3 subunits, PTK2, and talin and PTGER2/PTGER4 proteins through beta-arrestin-1 and Src kinase protein complex in human endometriotic epithelial cells 12Z and stromal cells 22B; and 4) decreases adhesion of 12Z and 22B cells to ECM collagen I, collagen IV, fibronectin, and vitronectin in a substrate-specific manner. These novel findings provide an important molecular framework for further evaluation of selective inhibition of PTGER2 and PTGER4 as potential nonsteroidal therapy to expand the spectrum of currently available treatment options for endometriosis in child-bearing age women.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Extracellular Matrix Integrins Receptors, Prostaglandin E, EP2 Subtype Receptors, Prostaglandin E, EP4 Subtype Arrestins Arrestins beta-Arrestin 1 beta-Arrestins Cell Adhesion Cell Line Dinoprostone Dinoprostone Endometriosis Endometriosis Extracellular Matrix Female Focal Adhesion Protein-Tyrosine Kinases Focal Adhesion Protein-Tyrosine Kinases Humans

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