Peroxisome proliferator-activated receptor-γ ligand inhibition of RANTES production by human endometriotic stromal cells is mediated through an upstream promoter element

Pritts EA, Elizabeth A. Pritts, Dong Zhao, Zhao D, Sae H Sohn, Sohn SH, Chao Va, Victor A. Chao, Leslie L. Waite, Waite LL, Taylor RN, Robert N. Taylor
Fertility and sterility · 2003 · vol. 80(2) , pp. 415–420 · doi:10.1016/s0015-0282(03)00600-9 · PMID:12909507 · W2121846529
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Peroxisome proliferator-activated receptor-γ ligand treatment inhibits RANTES production in human endometriotic stromal cells via an upstream promoter element.

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Abstract

ObjectiveTo investigate the effect of peroxisome proliferator activated receptor-gamma (PPAR-gamma) ligands on transcription and secretion of regulated upon activation normal T-cell expressed and secreted (RANTES) in endometriotic stromal cells.DesignControlled laboratory study.SettingAcademic research laboratory. Women in the follicular phase of the menstrual cycle undergoing laparoscopic resection for endometriosis. [1]. Transient transfection of endometriotic stromal cells with RANTES promoter vectors with and without a mutagenized PPAR-gamma response element (PPRE), then treatment with PPAR-gamma ligands; [2]. co-incubation of cells with PPAR-gamma ligands.Main outcome measure(s)RANTES promoter activity and RANTES secretion. In endometriotic stromal cells, addition of PPAR-gamma ligands (rosiglitazone and 15 deoxy-Delta(12,14) prostaglandin J(2)) inhibited RANTES promoter activity by 51% and 50%, respectively. In cells transfected with the same promoter after site-directed mutagenesis of the 5' PPRE, addition of PPAR-gamma ligands failed to inhibit promoter activity. When endometriotic stromal cells were treated with PPAR-gamma ligands, a decrease in RANTES secretion by 51% and 20%, respectively, was observed. CONCLUION(S): The PPAR-gamma ligands inhibit RANTES transcription and protein production in endometriotic stromal cells. Transcriptional repression appears to be mediated through a specific PPRE at -344 to -322 bp upstream from the RNA polymerase start site.

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Condition tags

endometriosis

MeSH descriptors

Chemokine CCL5 Chemokine CCL5 Endometriosis Promoter Regions, Genetic Receptors, Cytoplasmic and Nuclear Stromal Cells Thiazolidinediones Transcription Factors Base Sequence Base Sequence Cells, Cultured Chemokine CCL5 Endometriosis Endometriosis Female Humans Ligands Mutagenesis, Site-Directed Promoter Regions, Genetic Prostaglandin D2

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