Role of 8-iso-prostaglandin F2alpha and 25-hydroxycholesterol in the pathophysiology of endometriosis

Indu Sharma, Lakhbir Kaur Dhaliwal, Dhaliwal LK, Lakhbir Dhaliwal, Saha SC, Subhash Chand Saha, Sangwan S, Sonal Sangwan, Veena Dhawan, Dhawan V
Fertility and sterility · 2009 · vol. 94(1) , pp. 63–70 · doi:10.1016/j.fertnstert.2009.01.141 · PMID:19324352 · W2143446059
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This paper investigates the involvement of 8-iso-prostaglandin F2α and 25-hydroxycholesterol in the underlying mechanisms and progression of endometriosis.

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Abstract

OBJECTIVE: To investigate the involvement of 8-iso-PGF(2alpha) and 25-hydroxycholesterol (25-OH-Chol) in the pathophysiology of endometriosis. DESIGN: Observational case-control study using enzyme immunoassay and high-performance liquid chromatography (HPLC). SETTING: Postgraduate Institute of Medical Education and Research. PATIENT(S): Forty-five women undergoing laparoscopy (n = 25), laparotomy (n = 19), or tubal ligation (n =1). INTERVENTION(S): Venipuncture and laparoscopic peritoneal fluid (PF) collection. MAIN OUTCOME MEASURE(S): The levels of 8-iso-PGF(2alpha) were determined both in urine and PF of all the patients using enzyme immunoassay. The levels of 25-OH-Chol were determined by using reversed phase HPLC both in the plasma and PF samples. Oxidative damage to DNA was assessed by agarose gel electrophoresis. RESULT(S): Significantly increased levels of 8-iso-PGF(2alpha) were observed both in urine and PF of women with endometriosis compared with control women. Similarly, higher levels of 25-OH-Chol were observed both in plasma and PF of patients compared with controls and the difference was statistically significant. A clear-cut tailing pattern was observed in DNA of patients with endometriosis, indicating significant DNA damage. CONCLUSION(S): Our observations implicate oxidative stress in the pathophysiology of endometriosis. For the first time, we demonstrate that 8-iso-PGF(2alpha) and oxysterols (the known promoters of steroidogenesis) might be the culprits in this disease.

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Condition tags

endometriosis

MeSH descriptors

Dinoprost Endometriosis Hydroxycholesterols Adult Adult Ascitic Fluid Ascitic Fluid Ascitic Fluid Biomarkers Biomarkers Biomarkers Biomarkers Case-Control Studies Case-Control Studies Dinoprost Dinoprost Dinoprost Endometriosis Endometriosis Endometriosis

Citation neighborhood (2-hop)

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

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